Recombinant Rabbit Anti-CUL3 Antibody (clone R09-4D1) (CAT#: VS3-FY430)

Name: Recombinant Rabbit Anti-CUL3 Antibody (clone R09-4D1)
SKU: VS3-FY430
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes human, mouse and rat CUL3 protein. It can detect a band of approximately 89 kDa (observedd molecular weight: 89 kDa).

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-CUL3 Antibody (clone R09-4D1) in WB.

Figure 1. Recombinant Rabbit Anti-CUL3 Antibody (clone R09-4D1) in WB.

Western blot analysis of Cullin 3/CUL3 in K562, 3T3, Hela lysates using Cullin 3/CUL3 Antibody.

Normal Tissue

Figure 1 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong nuclear and granular cytoplasmic positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2678/ihc_selected.jpg

Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2678/6718_B_8_5.jpg

Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2678/6718_A_7_3.jpg

Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2678/6718_A_7_4.jpg

Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2678/6718_A_7_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2678/6718_A_5_6.jpg

Figure 7 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2678/6718_A_8_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000036257-CUL3

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic peptide of human Cullin 3

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat CUL3

Species Reactivity
Human, Mouse, Rat

Clone
R09-4D1

Applications
Western Blot, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 89 kDa; Observed MW: 89 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20

Target

Alternative Names
Cullin 3; CUL-3; Cullin-3; KIAA0617; PHA2E

Gene ID
8452

UniProt ID
Q13618

Secondary Antibody

Isotype Control

Related Resources

Related Signaling Pathways

Hedgehog Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT	Product Name	Application	Type
MOR-0875	Hi-Affi™ Rabbit Anti-CUL3 Recombinant Antibody (clone DS875AB)	WB, IP, FC	Rabbit IgG

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