Recombinant Rabbit Anti-CXCL8 Detection Antibody (clone R02-9M5)

Name: Recombinant Rabbit Anti-CXCL8 Detection Antibody (clone R02-9M5)
SKU: VS3-WK1691
Rating: 5 (1 reviews)

CAT#: VS3-WK1691

This antibody is a rabbit recombinant antibody against human CXCL8. It was developed by immunization of rabbit with recombinant human CXCL8 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R02-3Z9 and R02-9M5 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R02-9M5 can be used in ELISA analysis to identify human CXCL8.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/1010_F11_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human lymph node

Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in non germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line GAMG

Immunofluorescent staining of human cell line GAMG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/2058_E8_1_blue_red_green.jpg

Normal Tissue Figure 4 Kidney

Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_7_5.jpg

Normal Tissue Figure 5 Testis

Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_6_6.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_8_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (BJ hTERT+, GAMG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169429-CXCL8

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Specifications

Immunogen
Recombinant human CXCL8 protein

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human CXCL8

Species Reactivity
Human

Clone
R02-9M5

Applications
ELISA

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Aggregation
< 5%

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
0.01M PBS, pH 7.2

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Target

Alternative Names
C-X-C Motif Chemokine Ligand 8; Monocyte-Derived Neutrophil Chemotactic Factor; Monocyte-Derived Neutrophil-Activating Peptide; Granulocyte Chemotactic Protein 1; Chemokine (C-X-C Motif) Ligand 8; Beta Endothelial Cell-Derived Neutrophil Activating Peptide; Lung Giant Cell Carcinoma-Derived Chemotactic Protein; Lymphocyte Derived Neutrophil Activating Peptide; Alveolar Macrophage Chemotactic Factor I; Tumor Necrosis Factor-Induced Gene 1; Neutrophil-Activating Peptide 1; T-Cell Chemotactic Factor; Interleukin 8; Emoctakin; GCP-1; MDNCF; MONAP

Gene ID
3576

UniProt ID
P10145

Long Name
C-X-C Motif Chemokine Ligand 8

Sequence Similarities
Belongs to the intercrine alpha (chemokine CxC) family.

Cellular Localization
Secreted

Post Translation Modifications
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.

Protein Refseq
NP_000575.1

Function
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection.
Also plays an important role in neutrophil activation.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-WK1691.pdf

MSDS

MSDS - VS3-WK1691.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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