Recombinant Rabbit Anti-DDB1 Antibody (clone R07-2A3)
CAT#: VS3-FY446
This product is a recombinant rabbit antibody that recognizes human and mouse DDB1 protein. It can detect a band of approximately 127 kDa (observedd molecular weight: 127 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45174/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68456/if_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32821/63256_A_8_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32821/63256_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32821/63256_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32821/63256_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167986-DDB1
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WB
Figure 1. Recombinant Rabbit Anti-DDB1 Antibody (clone R07-2A3) in WB.
Western blot analysis of DDB1 in Hela, MCF-7, HepG2, U2OS, U251, HL-60 lysates using DDB1 Antibody.
IHC
Figure 2. Recombinant Rabbit Anti-DDB1 Antibody (clone R07-2A3) in IHC.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using DDB1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- A synthetic peptide of human DDB1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse DDB1
- Species Reactivity
- Human, Mouse
- Clone
- R07-2A3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 127 kDa; Observed MW: 127 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Damage Specific DNA Binding Protein 1; Xeroderma Pigmentosum Group E-Complementing Protein; UV-Damaged DNA-Binding Protein 1; UV-Damaged DNA-Binding Factor; DNA Damage-Binding Protein A; HBV X-Associated Protein 1; XPE-Binding Factor; DDB P127 Subunit; UV-DDB 1; XPE-BF; XAP-1
- Gene ID
- 1642
- UniProt ID
- Q16531
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DDB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0948 | Hi-Affi™ Rabbit Anti-DDB1 Recombinant Antibody (clone DS948AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0464-CN | Rabbit Anti-DDB1 Recombinant Antibody (clone JU32-35) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-1810-CN | Rabbit Anti-DDB1 Polyclonal Antibody (MRO-1810-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0474F | Mouse Anti-DDB1 Recombinant Antibody (ZG-0474F) | WB | Mouse IgG |
| VS3-QX318 | Mouse Anti-DDB1 Recombinant Antibody (clone 2D6-B5-E6) | WB | Mouse IgG2b |
| VS4-WK139 | Mouse Anti-DDB1 Recombinant Antibody (VS4-WK139) | ELISA, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC888 | AbPlus™ Anti-DDB1 Magnetic Beads (VS-0724-YC888) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY635 | Anti-DDB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1938 | Anti-Mouse DDB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1937 | Anti-Human DDB1 Immunohistochemistry Kit | IHC |
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