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  • Recombinant Rabbit Anti-DDX21 Antibody (clone R04-4J2)

Recombinant Rabbit Anti-DDX21 Antibody (clone R04-4J2) (CAT#: VS3-FY448)

This product is a recombinant rabbit antibody that recognizes human DDX21 protein. It can detect a band of approximately 87 kDa (observedd molecular weight: 87 kDa).


See more Engineered Antibody products for DDX21
Specific Inquiry
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  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/430_D9_1_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36593/592_E5_2_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/1888_B12_2_red_green.jpg
Normal Tissue
Figure 4 Colon
Figure 4 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_3.jpg
Figure 5 Kidney
Figure 5 Kidney
(Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_5.jpg
Figure 6 Skin
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_B_7_1.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165732-DDX21
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165732-DDX21

Specifications

  • Immunogen
  • A synthetic peptide of human DDX21
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human DDX21
  • Species Reactivity
  • Human
  • Clone
  • R04-4J2
  • Applications
  • Western Blot, Immunocytochemistry, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 87 kDa; Observed MW: 87 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95%
  • Format
  • Liquid
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • DExD-Box Helicase 21; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21; Nucleolar RNA Helicase Gu; Nucleolar RNA Helicase II; DEAD-Box Helicase 21; DEAD Box Protein 21; RH II/Gu; Gu-Alpha; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21; DEAD (Asp-Glu-Ala-Asp) Box Helicase 21

Recommended Products

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "DDX21"

Recombinant Antibody

CAT Product Name Application Type
MOB-1325z Mouse Anti-DDX21 Recombinant Antibody (clone 16A4) WB, IHC, IF, ELISA Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0957 Hi-Affi™ Rabbit Anti-DDX21 Recombinant Antibody (clone DS957AB) WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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