Recombinant Rabbit Anti-DDX21 Antibody (clone R04-4J2) (CAT#: VS3-FY448)
This product is a recombinant rabbit antibody that recognizes human DDX21 protein. It can detect a band of approximately 87 kDa (observedd molecular weight: 87 kDa).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/430_D9_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/430_D9_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36593/592_E5_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36593/592_E5_2_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/1888_B12_2_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/1888_B12_2_red_green.jpg
Figure 4 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_3.jpg
Figure 5 Kidney
(Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_5.jpg
(Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_5.jpg
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_B_7_1.jpg
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_B_7_1.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165732-DDX21
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165732-DDX21
Specifications
- Immunogen
- A synthetic peptide of human DDX21
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DDX21
- Species Reactivity
- Human
- Clone
- R04-4J2
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 87 kDa; Observed MW: 87 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- DExD-Box Helicase 21; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21; Nucleolar RNA Helicase Gu; Nucleolar RNA Helicase II; DEAD-Box Helicase 21; DEAD Box Protein 21; RH II/Gu; Gu-Alpha; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21; DEAD (Asp-Glu-Ala-Asp) Box Helicase 21
- Gene ID
- 9188
- UniProt ID
- Q9NR30
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1325z | Mouse Anti-DDX21 Recombinant Antibody (clone 16A4) | WB, IHC, IF, ELISA | Mouse IgG1 |
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