Recombinant Rabbit Anti-DGAT1 Antibody (clone R02-4G6)
CAT#: VS3-FY451
This product is a recombinant rabbit antibody that recognizes human DGAT1 protein. It can detect a band of approximately 55 kDa (observedd molecular weight: 55 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/971_F9_2_selected.jpg
Normal Tissue
Figure 2 Small intestine
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_4_2_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts Staining: Medium Intensity: Moderate Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Hep G2, PC-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185000-DGAT1
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WB
Figure 1. Recombinant Rabbit Anti-DGAT1 Antibody (clone R02-4G6) in WB.
Western blot analysis of DGAT1 in K562 lysates using DGAT1 antibody.
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Specifications
- Immunogen
- Recombinant protein of human DGAT1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DGAT1
- Species Reactivity
- Human
- Clone
- R02-4G6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 55 kDa; Observed MW: 55 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Diacylglycerol O-Acyltransferase 1; Acyl-CoA Retinol O-Fatty-Acyltransferase; Diglyceride Acyltransferase; EC 2.3.1.20; ARAT; DGAT; Acyl Coenzyme A:Cholesterol Acyltransferase Related Gene 1; Diacylglycerol O-Acyltransferase (Mouse) Homolog; Diacylglycerol O-Acyltransferase Homolog; Acyl-CoA:Diacylglycerol Acyltransferase
- Gene ID
- 8694
- UniProt ID
- O75907
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DGAT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0973 | Hi-Affi™ Rabbit Anti-DGAT1 Recombinant Antibody (clone DS973AB) | ICC, IF, IP, FC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC300 | Rabbit Anti-DGAT1 Recombinant Antibody (VS13-YC300) | WB, IHC-P, IHC-Fr, ICC, IF, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1979 | Anti-DGAT1 Immunohistochemistry Kit | IHC |
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