Recombinant Rabbit Anti-DGAT1 Antibody (clone R02-4G6) (CAT#: VS3-FY451)

Name: Recombinant Rabbit Anti-DGAT1 Antibody (clone R02-4G6)
SKU: VS3-FY451
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes human DGAT1 protein. It can detect a band of approximately 55 kDa (observedd molecular weight: 55 kDa).

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-DGAT1 Antibody (clone R02-4G6) in WB.

Figure 1. Recombinant Rabbit Anti-DGAT1 Antibody (clone R02-4G6) in WB.

Western blot analysis of DGAT1 in K562 lysates using DGAT1 antibody.

Subcellular Location

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/971_F9_2_selected.jpg

Normal Tissue

Figure 2 Small intestine
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_4_2_val_selected.jpg

Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_B_9_5.jpg

Figure 4 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_8_3.jpg

Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_9_4.jpg

Figure 6 Kidney
(Collecting ducts Staining: Medium Intensity: Moderate Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_8_5.jpg

Figure 7 Testis
(Sertoli cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32853/73790_A_5_6.jpg

RNA Expression

Figure 8 RNA cell line category: Cell line enhanced (Hep G2, PC-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185000-DGAT1

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Recombinant protein of human DGAT1

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human DGAT1

Species Reactivity
Human

Clone
R02-4G6

Applications
Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 55 kDa; Observed MW: 55 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20

Target

Alternative Names
Diacylglycerol O-Acyltransferase 1; Acyl-CoA Retinol O-Fatty-Acyltransferase; Diglyceride Acyltransferase; EC 2.3.1.20; ARAT; DGAT; Acyl Coenzyme A:Cholesterol Acyltransferase Related Gene 1; Diacylglycerol O-Acyltransferase (Mouse) Homolog; Diacylglycerol O-Acyltransferase Homolog; Acyl-CoA:Diacylglycerol Acyltransferase

Gene ID
8694

UniProt ID
O75907

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody Products IgG Antibody Products

CAT	Product Name	Application	Type
MOR-0973	Hi-Affi™ Rabbit Anti-DGAT1 Recombinant Antibody (clone DS973AB)	ICC, IF, IP, FC, WB	Rabbit IgG

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