Recombinant Rabbit Anti-EIF4A1 Antibody (clone R04-2K6) (CAT#: VS3-FY485)

This product is a recombinant rabbit antibody that recognizes human, mouse and rat EIF4A1 protein. It can detect a band of approximately 46 kDa (observedd molecular weight: 46 kDa).


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WB

Figure 1. Recombinant Rabbit Anti-EIF4A1 Antibody (clone R04-2K6) in WB.

Figure 1. Recombinant Rabbit Anti-EIF4A1 Antibody (clone R04-2K6) in WB.

Western blot analysis of eIF4A1 in K562, C6, 3T3, Hela lysates using eIF4A1 Antibody.


Specifications

  • Immunogen
  • A synthetic peptide of human eIF4A1
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat EIF4A1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R04-2K6
  • Applications
  • Western Blot, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 46 kDa; Observed MW: 46 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95%
  • Format
  • Liquid
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Eukaryotic Translation Initiation Factor 4A1; ATP-Dependent RNA Helicase EIF4A-1; EIF-4A-I; EIF4A-I; EIF4A; DDX2A; Eukaryotic Translation Initiation Factor 4A, Isoform 1
  • Sequence Similarities
  • Belongs to the DEAD box helicase family. eIF4A subfamily.
  • Cellular Localization
  • Cytoplasm, Cytosol
  • Function
  • ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "EIF4A1"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1125 Hi-Affi™ Rabbit Anti-EIF4A1 Recombinant Antibody (clone DS1125AB) WB, ICC, IF, FC Rabbit IgG

Recombinant Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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