Recombinant Rabbit Anti-ELK1 Antibody (clone R06-5J2)
CAT#: VS3-FY493
This product is a recombinant rabbit antibody that recognizes human ELK1 protein. It can detect a band of approximately 45 kDa (observedd molecular weight: 62 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64381/1164_E3_1_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3808/if_selected.jpg
Normal Tissue
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3808/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_A_4_6.jpg
Normal Tissue
Figure 6 Ovary
Ovarian stroma cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_A_5_7.jpg
Normal Tissue
Figure 7 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_B_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126767-ELK1
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WB
Figure 1. Recombinant Rabbit Anti-ELK1 Antibody (clone R06-5J2) in WB.
Western blot analysis of ELK1 in HeLa lysates using ELK1 antibody.
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Specifications
- Immunogen
- A synthetic peptide of human ELK1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ELK1
- Species Reactivity
- Human
- Clone
- R06-5J2
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Chromatin Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 45 kDa; Observed MW: 62 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Chimmunoprecipitation: 1/20
Target
- Alternative Names
- ELK1, ETS Transcription Factor; ELK1, Member Of ETS Oncogene Family
- Gene ID
- 2002
- UniProt ID
- P19419
- Sequence Similarities
- Belongs to the ETS family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).
- Protein Refseq
- NP_001107595.1; NP_001244097.1; NP_005220.2
- Function
- Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).
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Related Signaling Pathways
cAMP Signaling Pathway
ErbB Signaling Pathway
MAPK Signaling Pathway
Ras Signaling Pathway
Related Diseases
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Throid Cancer
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ELK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1138 | Hi-Affi™ Rabbit Anti-ELK1 Recombinant Antibody (clone DS1138AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0530-CN | Rabbit Anti-ELK1 Recombinant Antibody (clone CBACN-212) | WB, IF, IP | Rabbit IgG |
| VS3-CJ747 | Rabbit Anti-ELK1 Recombinant Antibody (VS3-CJ747) | WB, ICC, IP | Rabbit IgG |
| VS3-FY494 | Recombinant Rabbit Anti-ELK1 Antibody (clone R08-6K4) | WB | Rabbit IgG |
| VS7-HM857 | Mouse Anti-ELK1 Recombinant Antibody (clone 3H6D12) | IHC | Mouse IgG1 |
| VS7-HM859 | Mouse Anti-ELK1 Recombinant Antibody (clone CBL225HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC160 | AbPlus™ Anti-ELK1 Magnetic Beads (VS-0724-YC160) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2251 | Anti-ELK1 Immunohistochemistry Kit | IHC |
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