Recombinant Mouse Anti-ENO1 Detection Antibody (clone 6H2)
CAT#: VS3-WK1659
This antibody is a mouse recombinant antibody against human ENO1. The sandwich ELISA was established using matched antibody pair 9F10 and 6H2. The clone 6H2 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human ENO1 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
Normal Tissue
Figure 10 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
Normal Tissue
Figure 11 Pancreas
Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
Normal Tissue
Figure 12 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
Normal Tissue
Figure 13 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
Normal Tissue
Figure 14 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
RNA Expression
Figure 15 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1
❮
❯
Specifications
- Immunogen
- Recombinant human ENO1 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ENO1
- Species Reactivity
- Human
- Clone
- 6H2
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Enolase 1; 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; Alpha-Enolase; EC 4.2.1.11; ENO1L1; MPB1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1
- Gene ID
- 2023
- UniProt ID
- P06733
- Long Name
- Enolase 1
- Sequence Similarities
- Belongs to the enolase family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- ISGylated.
- Protein Refseq
- NP_001188412.1; NP_001419.1
- Function
- Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons.
Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1162z | Mouse Anti-ENO1 Recombinant Antibody (clone 28F5) | WB, ELISA, IF, IHC | Mouse IgG1, κ |
| FAMAB-0235-CN | Chicken Anti-ENO1 Recombinant Antibody (clone EnL2) | ELISA, WB, IF, FC | Chicken IgY |
| FAMAB-0236-CN | Chicken Anti-ENO1 Recombinant Antibody (clone EnL5) | ELISA, WB, IF, FC | Chicken IgY |
| HPAB-A1275-YJ | Chicken Anti-ENO1 Recombinant Antibody (clone EnL1) | WB, ELISA | Chicken IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-350MZ | Anti-Human ENO1 Recombinant Antibody (A3) | WB, FC | Human antibody |
| TAB-350MZ-S(P) | Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) | WB, FC | Human antibody |
| TAB-350MZ-F(E) | Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) | WB, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0190MZ | Chicken Anti-ENO1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC842 | PE-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
| MHC-LC843 | APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
| MHC-LC844 | BV421-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1151 | Hi-Affi™ Rabbit Anti-ENO1 Recombinant Antibody (clone DS1151AB) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0054-CN-S(P) | Mouse Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0054-CN-S(P)) | FC, ELISA | Mouse scFv |
| HPAB-0055-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0055-CN-S(P)) | FC, ELISA | Humanized scFv |
| HPAB-0056-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0056-CN-S(P)) | FC, ELISA | Humanized scFv |
| HPAB-0057-CN-S(P) | Chicken Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0057-CN-S(P)) | FC, WB, ELISA, IF | Chicken scFv |
| HPAB-0058-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0058-CN-S(P)) | FC, WB, ELISA, IF | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0054-CN-F(E) | Mouse Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0054-CN-F(E)) | FC, ELISA | Mouse Fab |
| HPAB-0055-CN-F(E) | Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0055-CN-F(E)) | FC, ELISA | Humanized Fab |
| HPAB-0056-CN-F(E) | Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0056-CN-F(E)) | FC, ELISA | Humanized Fab |
| HPAB-0057-CN-F(E) | Chicken Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0057-CN-F(E)) | FC, WB, ELISA, IF | Chicken Fab |
| HPAB-0058-CN-F(E) | Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0058-CN-F(E)) | FC, WB, ELISA, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF036 | DRB1*04:01/Human cit alpha-enolase (IFDS[cit]GNPTVEVDLF) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1382 | AbPlus™ Anti-Eno1 Magnetic Beads (VS-0724-YC1382) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY767 | Anti-ENO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2287 | Anti-Mouse ENO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2286 | Anti-Human ENO1 Immunohistochemistry Kit | IHC |
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