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  • Recombinant Mouse Anti-ENO1 Detection Antibody (clone 6H2)

Recombinant Mouse Anti-ENO1 Detection Antibody (clone 6H2) (CAT#: VS3-WK1659)

This antibody is a mouse recombinant antibody against human ENO1. The sandwich ELISA was established using matched antibody pair 9F10 and 6H2. The clone 6H2 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human ENO1 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Figure 3 IF staining of human cell line A549
Figure 3 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
Figure 4 IHC staining of human pancreas
Figure 4 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
Figure 5 IHC staining of human kidney
Figure 5 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows moderate positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
Figure 6 IHC staining of human kidney
Figure 6 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
Figure 7 IF staining of human cell line MCF7
Figure 7 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Figure 8 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
Figure 9 Colon
Figure 9 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
Figure 10 Liver
Figure 10 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
Figure 11 Pancreas
Figure 11 Pancreas
(Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
Figure 12 Kidney
Figure 12 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
Figure 13 Testis
Figure 13 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
Figure 14 Lymph node
Figure 14 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
RNA Expression
Figure 15 RNA cell line category: Low cell line specificity
Figure 15 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1

Specifications

  • Immunogen
  • Recombinant human ENO1 protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human ENO1
  • Species Reactivity
  • Human
  • Clone
  • 6H2
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.01% thimerosal
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • ELISA: 1/1000

Target

  • Alternative Names
  • Enolase 1; 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; Alpha-Enolase; EC 4.2.1.11; ENO1L1; MPB1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1
  • Long Name
  • Enolase 1
  • Sequence Similarities
  • Belongs to the enolase family.
  • Cellular Localization
  • Cell membrane, Cytoplasm, Membrane, Nucleus
  • Post Translation Modifications
  • ISGylated.
  • Function
  • Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons.
    Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ENO1"

Recombinant Antibody

CAT Product Name Application Type
MOB-1162z Mouse Anti-ENO1 Recombinant Antibody (clone 28F5) WB, ELISA, IF, IHC Mouse IgG1, κ
MOB-0661CT Recombinant Mouse anti-Human ENO1 Monoclonal antibody (3H3BH22CG9) FC, ICC/IF, IP, ELISA
HPAB-0054-CN Mouse Anti-ENO1 Recombinant Antibody (HPAB-0054-CN) FC, ELISA Mouse IgG
HPAB-0055-CN Human Anti-ENO1 Recombinant Antibody (HPAB-0055-CN) FC, ELISA Humanized IgG
HPAB-0056-CN Human Anti-ENO1 Recombinant Antibody (HPAB-0056-CN) FC, ELISA Humanized IgG

Human Antibody

CAT Product Name Application Type
TAB-350MZ Anti-Human ENO1 Recombinant Antibody (A3) WB, FC Human antibody
TAB-350MZ-S(P) Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) WB, FC Human antibody
TAB-350MZ-F(E) Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) WB, FC Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0190MZ Chicken Anti-ENO1 Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC842 PE-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer FCM
MHC-LC843 APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer FCM
MHC-LC844 BV421-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer FCM

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1151 Hi-Affi™ Rabbit Anti-ENO1 Recombinant Antibody (clone DS1151AB) WB, ICC, IF, IP Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0054-CN-F(E) Mouse Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0054-CN-F(E)) FC, ELISA Mouse Fab
HPAB-0055-CN-F(E) Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0055-CN-F(E)) FC, ELISA Humanized Fab
HPAB-0056-CN-F(E) Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0056-CN-F(E)) FC, ELISA Humanized Fab
FAMAB-0235-CN-F(E) Chicken Anti-ENO1 Recombinant Antibody (clone EnL2); Fab Fragment ELISA, WB, IF, FC Chicken Fab
FAMAB-0236-CN-F(E) Chicken Anti-ENO1 Recombinant Antibody (clone EnL5); Fab Fragment ELISA, WB, IF, FC Chicken Fab

scFv Fragment Antibody

CAT Product Name Application Type
FAMAB-0235-CN-S(P) Chicken Anti-ENO1 Recombinant Antibody (clone EnL2); scFv Fragment ELISA, WB, IF, FC Chicken scFv
FAMAB-0236-CN-S(P) Chicken Anti-ENO1 Recombinant Antibody (clone EnL5); scFv Fragment ELISA, WB, IF, FC Chicken scFv
HPAB-J0072-YC-S(P) Human Anti-ENO1 Recombinant Antibody (clone B3); scFv Fragment ELISA Human scFv
HPAB-J0073-YC-S(P) Human Anti-ENO1 Recombinant Antibody (clone C4); scFv Fragment ELISA Human scFv
HPAB-J0074-YC-S(P) Human Anti-ENO1 Recombinant Antibody (clone B8); scFv Fragment ELISA Human scFv

MHC Tetramer for Other Applications

CAT Product Name Application Type
MHC-YF036 DRB1*04:01/Human cit alpha-enolase (IFDS[cit]GNPTVEVDLF) MHC Monomer MHC Multimer

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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