Recombinant Rabbit Anti-ENO3 Antibody (clone R07-5B2)
CAT#: VS3-FY499
This product is a recombinant rabbit antibody that recognizes human, mouse and rat ENO3 protein. It can detect a band of approximately 47 kDa (observedd molecular weight: 47 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/793/26_C2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/793/2247_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/793/2247_A_8_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/793/2247_A_6_6.jpg
Normal Tissue
Figure 6 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/793/2247_B_1_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, HeLa, HMC-1, RH-30, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108515-ENO3
❮
❯
WB
Figure 1. Recombinant Rabbit Anti-ENO3 Antibody (clone R07-5B2) in WB.
Western blot analysis of ENO3 in rat brain, C6, 3T3, Hela lysates using ENO3 Antibody.
❮
❯
Specifications
- Immunogen
- A synthetic peptide of human ENO3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ENO3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-5B2
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 47 kDa; Observed MW: 47 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY499. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ENO3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2369z | Mouse Anti-ENO3 Recombinant Antibody (clone 42A5) | WB, ELISA, ICC, IF, IHC | Mouse IgG2a, κ |
| MOB-0662CT | Recombinant Mouse anti-Human ENO3 Monoclonal antibody (EML305) | IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1153 | Hi-Affi™ Rabbit Anti-ENO3 Recombinant Antibody (clone DS1153AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC320 | AbPlus™ Anti-ENO3 Magnetic Beads (VS-0724-YC320) | IP, Protein Purification | |
| VS-0724-YC407 | AbPlus™ Anti-ENO3 Magnetic Beads (VS-0724-YC407) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY768 | Anti-ENO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2292 | Anti-Rat ENO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2291 | Anti-Human ENO3 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
-2.png)
Application: IB, ELISA, FC, FuncS
![Figure 2 Anti-Human CD19 Recombinant Antibody Fab Fragment [TAB-1611CL-F(E)] in HPLC](https://img.creativebiolabs.net/productimages/COA-TAB-1611CL-F(E)-2.png)
Application: Depletion, FuncS
Application: FC, FRET, Internalization
Application: WB, ELISA, Neut, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
