Recombinant Mouse Anti-ERCC1 Antibody (clone 5A2C3) (CAT#: VS3-FY517)
This product is a recombinant mouse antibody that recognizes ERCC1 protein. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50182/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50182/if_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72859/157426_A_4_1_selected.jpg
(Immunohistochemical staining of human stomach shows nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72859/157426_A_4_1_selected.jpg
Figure 3 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_9_5.jpg
Figure 4 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_5_6.jpg
Figure 5 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_B_5_6.jpg
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_B_5_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_8_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_8_8.jpg
Figure 7 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_B_7_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000012061-ERCC1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000012061-ERCC1
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ERCC1
- Clone
- 5A2C3
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95%
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry.
Target
- Alternative Names
- ERCC Excision Repair 1, Endonuclease Non-Catalytic Subunit; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 (Includes Overlapping Antisense Sequence); Excision Repair Cross-Complementation Group 1; DNA Excision Repair Protein ERCC-1; COFS4; RAD10; UV20
- Gene ID
- 2067
- UniProt ID
- P07992
- Sequence Similarities
- Belongs to the ERCC1/RAD10/SWI10 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitinated with both 'Lys-48' and 'Lys-63' linkages (PubMed:25538220).
Deubiquitinated by USP45 (PubMed:25538220).
- Protein Refseq
- NP_001159521.1; NP_001974.1; NP_973730.1
- Function
- Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1931z | Mouse Anti-ERCC1 Recombinant Antibody (clone 21G8) | WB, ICC, IF, IHC | Mouse IgG1 |
| MOB-0690CT | Recombinant Mouse anti-Human ERCC1 Monoclonal antibody (3437D2b) | Dot, WB | |
| ZG-0020C | Mouse Anti-ERCC1 Recombinant Antibody (clone 1B10) | WB, IHC-P, IF | Mouse IgG |
| ZG-0021C | Mouse Anti-ERCC1 Recombinant Antibody (clone PT0756) | WB, IF/ICC | Mouse IgG |
| VS3-XY648 | Mouse Anti-ERCC1 Recombinant Antibody (clone 1H10D2) | ELISA, WB, ICC, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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