Recombinant Mouse Anti-ERCC1 Antibody (VS3-FY519)
CAT#: VS3-FY519
This product is a recombinant mouse antibody that recognizes human ERCC1. ERCC1 mouse Monoclonal antibody detects endogenous ERCC1 protein protein. It can detect a band of approximately 39 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50182/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72859/157426_A_4_1_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_9_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_5_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_B_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_8_8.jpg
Normal Tissue
Figure 7 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_B_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000012061-ERCC1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- ERCC1 Mouse Monoclonal antibody detects endogenous ERCC1 protein.
- Species Reactivity
- Human
- Applications
- Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated
- MW
- 39 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western blotting: 1/1000-1/2000
Target
- Alternative Names
- ERCC Excision Repair 1, Endonuclease Non-Catalytic Subunit; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 (Includes Overlapping Antisense Sequence); Excision Repair Cross-Complementation Group 1; DNA Excision Repair Protein ERCC-1; COFS4; RAD10; UV20
- Gene ID
- 2067
- UniProt ID
- P07992
- Sequence Similarities
- Belongs to the ERCC1/RAD10/SWI10 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitinated with both 'Lys-48' and 'Lys-63' linkages (PubMed:25538220).
Deubiquitinated by USP45 (PubMed:25538220).
- Protein Refseq
- NP_001159521.1; NP_001974.1; NP_973730.1
- Function
- Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ERCC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0690CT | Recombinant Mouse anti-Human ERCC1 Monoclonal antibody (3437D2b) | Dot, WB | |
| VS3-CJ482 | Mouse Anti-ERCC1 Recombinant Antibody (VS3-CJ482) | WB, IHC | Mouse IgG |
| VS4-WK165 | Mouse Anti-ERCC1 Recombinant Antibody (VS4-WK165) | ELISA, WB | Mouse IgG |
| VS7-HM895 | Mouse Anti-ERCC1 Recombinant Antibody (clone 1A5A2) | FCM | Mouse IgG1 |
| VS7-HM896 | Mouse Anti-ERCC1 Recombinant Antibody (clone 1E5B3) | ICC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1183 | Hi-Affi™ Rabbit Anti-ERCC1 Recombinant Antibody (clone DS1183AB) | FC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2341 | Anti-ERCC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2342 | Anti-Mouse ERCC1 Immunohistochemistry Kit | IHC |
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