Recombinant Mouse Anti-ERCC1 Antibody (VS3-FY519) (CAT#: VS3-FY519)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• ERCC1 Mouse Monoclonal antibody detects endogenous ERCC1 protein.

• Species Reactivity
• Human

• Applications
• Western Blot, Immunohistochemistry

• Conjugate
• Unconjugated

• MW
• 39 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• PBS (without Mg^{2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% Glycerol}

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western blotting: 1/1000-1/2000

• Alternative Names
• ERCC Excision Repair 1, Endonuclease Non-Catalytic Subunit; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 (Includes Overlapping Antisense Sequence); Excision Repair Cross-Complementation Group 1; DNA Excision Repair Protein ERCC-1; COFS4; RAD10; UV20

• Gene ID
• 2067

• UniProt ID
• P07992

• Sequence Similarities
• Belongs to the ERCC1/RAD10/SWI10 family.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Ubiquitinated with both 'Lys-48' and 'Lys-63' linkages (PubMed:25538220).
  Deubiquitinated by USP45 (PubMed:25538220).

• Protein Refseq
• NP_001159521.1; NP_001974.1; NP_973730.1

• Function
• Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.

Secondary Antibody

• 2ND-037CQ
• Chicken Anti-Mouse IgG (H&L) Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-041CQ
• Llama Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Select a product category from the dropdown menu below to view related products.