Recombinant Rabbit Anti-ERCC2 Antibody (clone R08-2I3)
CAT#: VS3-FY520
This product is a recombinant rabbit antibody that recognizes human ERCC2 protein. It can detect a band of approximately 87 kDa (observedd molecular weight: 80 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69266/1606_F6_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104884-ERCC2
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WB
Figure 1. Recombinant Rabbit Anti-ERCC2 Antibody (clone R08-2I3) in WB.
Western blot analysis of XPD in Jurkat, Hela lysates using XPD Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human XPD
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ERCC2
- Species Reactivity
- Human
- Clone
- R08-2I3
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 87 kDa; Observed MW: 80 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- ERCC Excision Repair 2, TFIIH Core Complex Helicase Subunit; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2; TFIIH Basal Transcription Factor Complex Helicase XPB Subunit; TFIIH Basal Transcription Factor Complex 80 KDa Subunit; Xeroderma Pigmentosum Group D-Complementing Protein; Excision Repair Cross-Complementation Group 2; Xeroderma Pigmentosum Complementary Group D; Basic Transcription Factor 2 80 KDa Subunit; DNA Repair Protein Complementing XP-D Cells; DNA Excision Repair Protein ERCC-2; TFIIH 80 KDa Subunit; TFIIH P80; BTF2 P80; CXPD
- Gene ID
- 2068
- UniProt ID
- P18074
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ERCC2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1793MZ | Recombinant Mouse Anti-Human ERCC2 Antibody | FC, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1184 | Hi-Affi™ Rabbit Anti-ERCC2 Recombinant Antibody (clone DS1184AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY781 | Anti-ERCC2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2343 | Anti-Human ERCC2 Immunohistochemistry Kit | IHC |
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