Recombinant Rabbit Anti-EZH2 Antibody (clone R07-5B1)
CAT#: VS3-FY526
This product is a recombinant rabbit antibody that recognizes EZH2. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The antigen is synthetic peptide of human KMT6/EZH2. The clone R07-5B1 is specific for human, mouse, rat EZH2.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2008_C2_16_cr5f1a9e9d6c53d_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2027_D2_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/582_H8_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106462-EZH2
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WB
Figure 1. Recombinant Rabbit Anti-EZH2 Antibody (clone R07-5B1) in WB.
Western Blot analysis of KMT6/EZH2 in C6, 3T3 lysates using KMT6 Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human KMT6/EZH2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat EZH2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-5B1
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 85 kDa; Observed MW: 98 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A; EZH2
- Gene ID
- 2146
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EZH2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1221 | Hi-Affi™ Rabbit Anti-EZH2 Recombinant Antibody (clone DS1221AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0657 | PE-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0658 | APC-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0659 | FITC-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0660 | A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-YF430 | A*02:01/Human EZH2 (SQADALKYV) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX394 | Mouse Anti-EZH2 Recombinant Antibody (clone 3D2A4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX395 | Mouse Anti-EZH2 Recombinant Antibody (clone 144CT2.1.1.5) | WB, ICC, IHC | Mouse IgG1, κ |
| VS3-QX396 | Mouse Anti-EZH2 Recombinant Antibody (clone 6F-4G) | WB, IHC, FC | Mouse IgG1 |
| VS3-WK1001 | Rabbit Anti-EZH2 Recombinant Antibody (VS3-WK1001) | WB, ICC, IF, IHC | Rabbit IgG |
| VS13-YC368 | Rabbit Anti-EZH2 Recombinant Antibody (VS13-YC368) | IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY197 | Mouse Anti-NHP EZH2 Recombinant Antibody (clone H.547.3) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2396 | Anti-EZH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2397 | Anti-Mouse EZH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2398 | Anti-Rat EZH2 Immunohistochemistry Kit | IHC |
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