Recombinant Rabbit Anti-GAD1 Capture Antibody (clone R77-2B-4) (CAT#: VS3-WK1836)
This antibody is a rabbit recombinant antibody against human GAD1. It was developed by immunization of rabbit with recombinant human GAD1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R77-2B-4 and R48-1J-6. The clone R77-2B-4 can be used as a capture antibody in ELISA analysis to identify human GAD1.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48871/1825_E5_41_cr5ac3411596194_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48871/1825_E5_41_cr5ac3411596194_selected.jpg
Figure 2 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48871/865_B12_7_blue_green.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48871/865_B12_7_blue_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48871/783_B4_1_blue_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48871/783_B4_1_blue_green.jpg
Figure 4 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58412/134088_B_8_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58412/134088_B_8_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, HEL)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128683-GAD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128683-GAD1
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, HEL)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128683-GAD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128683-GAD1
Specifications
- Immunogen
- Recombinant human GAD1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GAD1
- Species Reactivity
- Human
- Clone
- R77-2B-4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Glutamate Decarboxylase 1; Glutamate Decarboxylase 1 (Brain, 67kDa); Glutamate Decarboxylase 67 KDa Isoform; 7 KDa Glutamic Acid Decarboxylase; EC 4.1.1.15; GAD-67; GAD
- Gene ID
- 2571
- UniProt ID
- Q99259
- Long Name
- Glutamate Decarboxylase 1
- Sequence Similarities
- Belongs to the group II decarboxylase family.
- Cellular Localization
- Axon terminus, Cell cortex, Cytoplasm
- Protein Refseq
- NP_000808.2
- Function
- Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.
Recommended Products
Detection antibody
Isotype Control
Secondary Antibody
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GAD1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1103z | Mouse Anti-GAD1 Recombinant Antibody (clone 26H4) | WB, ICC, IF, IHC, IP | Mouse IgG2b |
| MOB-2429MZ | Recombinant Mouse Anti-Human GAD1 Antibody (clone 6E9) | IHC-P, WB | Mouse antibody |
| MRO-0619-CN | Rabbit Anti-GAD1 Recombinant Antibody (clone JM11-11) | WB, IP | Rabbit IgG |
| VS3-WK1837 | Recombinant Rabbit Anti-GAD1 Detection Antibody (clone R48-1J-6) | ELISA | Rabbit IgG |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0697MZ | Chicken Anti-GAD1 Polyclonal IgY | ICC/IF, IHC, WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1388 | Hi-Affi™ Rabbit Anti-GAD1 Recombinant Antibody (clone DS1388AB) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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