Recombinant Rabbit Anti-GBP1 Antibody (clone R02-7B6)
CAT#: VS3-FY581
This product is a recombinant rabbit antibody that recognizes GBP1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R02-7B6 is specific for human GBP1. The antigen is human gbp1 synthetic peptide.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-GBP1 Antibody (clone R02-7B6) in WB.
Western Blot analysis of GBP1 in HepG2, U251, HL-60, Lncap lysates using GBP1 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-GBP1 Antibody (clone R02-7B6) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using GBP1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells along with distinctly stained extracellular material.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15450/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15450/144732_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15450/144732_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15450/144732_A_7_5.jpg
Normal Tissue
Figure 5 Appendix
Lymphoid tissue Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15450/144732_A_2_2.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, GAMG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117228-GBP1
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Specifications
- Immunogen
- Human GBP1 Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GBP1
- Species Reactivity
- Human
- Clone
- R02-7B6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 68 kDa; Observed MW: 68 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Related Diseases
Gastric Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GBP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0599CT | Recombinant Mouse anti-Human GBP1 Monoclonal antibody (5E20) | ELISA, IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1410 | Hi-Affi™ Rabbit Anti-GBP1 Recombinant Antibody (clone DS1410AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC655 | AbPlus™ Anti-GBP1 Magnetic Beads (VS-0724-YC655) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY903 | Anti-GBP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2756 | Anti-Human GBP1 Immunohistochemistry Kit | IHC |
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