Recombinant Rabbit Anti-HCK Antibody (clone R02-5C1)
CAT#: VS3-FY657
This product is a recombinant rabbit antibody that recognizes HCK. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-5C1 is specific for human HCK. The antigen is human hck synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35534/2069_B3_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63768/2005_C2_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows cytoplasmic positivity in syncytiotrophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/13714_B_7_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/13714_A_8_4.jpg
Normal Tissue
Figure 6 Testis
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/13714_A_6_6.jpg
Normal Tissue
Figure 7 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/13714_B_6_4.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (THP-1, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101336-HCK
❮
❯
ICC
Figure 1. Recombinant Rabbit Anti-HCK Antibody (clone R02-5C1) in ICC.
Immunocytochemical analysis of Hck (green) in hela using Hck antibody and DAPI (blue).
❮
❯
Specifications
- Immunogen
- Human Hck synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HCK
- Species Reactivity
- Human
- Clone
- R02-5C1
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 60 kDa; Observed MW: 60 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- HCK Proto-Oncogene, Src Family Tyrosine Kinase; Hemopoietic Cell Kinase; Hematopoietic Cell Kinase; P59-HCK/P60-HCK; EC 2.7.10.2; P59Hck
- Gene ID
- 3055
- UniProt ID
- P08631
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Lysosome, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases.
Ubiquitinated by CBL, leading to its degradation via the proteasome.
Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.
- Protein Refseq
- NP_001165600.1; NP_001165601.1; NP_001165602.1
- Function
- Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY657. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HCK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3439z | Mouse Anti-HCK Recombinant Antibody (clone 40H4) | WB, IP, IF | Mouse IgG1 |
| ZG-0410J | Mouse Anti-HCK Recombinant Antibody (ZG-0410J) | WB, IHC, ELISA | Mouse IgG |
| VS3-CJ511 | Mouse Anti-HCK Recombinant Antibody (VS3-CJ511) | WB, ICC | Mouse IgG1, κ |
| VS3-FY2732 | Mouse Anti-HCK Recombinant Antibody (VS3-FY2732) | WB, ICC | Mouse IgG |
| VS7-0425-WR18 | Mouse Anti-HCK Monoclonal Antibody (VS7-0425-WR18) | WB, IHC-P, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1570 | Rabbit Anti-HCK Recombinant Antibody (clone DS1570AB) | FC, ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3080 | Anti-HCK Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, IHC, FC, Cyt, ELISA
Application: WB, ELISA, FuncS
Application: FuncS, Inhib, IP, ELISA
-CB2006C17L-4.jpg)
Application: WB, ELISA
Application: FC, FRET, Internalization
-5.jpg)
Application: ELISA, FC, Inhib
Application: Neut, IHC, Activ, FuncS, IF, ELISA
Application: ELISA, Inhib, FC, Neut
Application: FC
Application: ELISA, Block, WB, FC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
