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Recombinant Rabbit Anti-HIST1H3A Antibody (clone R06-2C5)

Recombinant Rabbit Anti-HIST1H3A Antibody (clone R06-2C5) (CAT#: VS3-FY646)

Name: Recombinant Rabbit Anti-HIST1H3A Antibody (clone R06-2C5)
SKU: VS3-FY646
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes HIST1H3A. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R06-2C5 is specific for human, mouse HIST1H3A. The antigen is synthetic peptide derived from human histone h3 (acetyl k4).

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-HIST1H3A Antibody (clone R06-2C5) in WB.

Figure 1. Recombinant Rabbit Anti-HIST1H3A Antibody (clone R06-2C5) in WB.

Western Blot analysis of acetyl-histone H3 (Lys4) in 3T3 lysates using an acetyl-histone H3 (Lys4) antibody.

Figure 2. Recombinant Rabbit Anti-HIST1H3A Antibody (clone R06-2C5) in WB.

Figure 2. Recombinant Rabbit Anti-HIST1H3A Antibody (clone R06-2C5) in WB.

Western Blot analysis of Acetyl-Histone H3 (Lys4) in A549, HL-60 lysates using Acetyl-Histone H3 (Lys4) Antibody.

Normal Tissue

Figure 1 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_B_7_5.jpg

Figure 2 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_3.jpg

Figure 3 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_7_4.jpg

Figure 4 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_9_5.jpg

Figure 5 Testis
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_4_6.jpg

Figure 6 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_8.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enhanced (U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000275714-H3C1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide derived from human histone H3 (acetyl K4).

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse HIST1H3A

Species Reactivity
Human, Mouse

Clone
R06-2C5

Applications
Western Blot, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 15 kDa; Observed MW: 15 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200

Target

Alternative Names
Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3/A; H3FA; HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J; Histone H3.1; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I

Gene ID
8350

UniProt ID
P68431

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products

CAT	Product Name	Application	Type
VS3-FY645	Recombinant Rabbit Anti-HIST1H3A (phospho Ser28) Antibody (clone R03-7K5)	WB, IHC-Fr, IHC-P, ICC, IF	Rabbit IgG
VS3-FY647	Recombinant Rabbit Anti-HIST1H3A Antibody (clone R06-4I8)	WB, IHC-P	Rabbit IgG
VS3-FY648	Recombinant Rabbit Anti-HIST1H3A Antibody (clone R08-4A0)	WB, IHC-Fr, IHC-P, ICC, IF, IP, ChIP	Rabbit IgG
VS3-WK1574	Mouse Anti-H3C1 Recombinant Antibody (VS3-WK1574)	WB	Mouse IgG
VS3-WK1576	Mouse Anti-H3C1 Recombinant Antibody (clone 8F7)	WB	Mouse IgG

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