Recombinant Rabbit Anti-HIST1H3A (phospho Thr3) Antibody (clone R08-9D6)

CAT#: VS3-FY650

This product is a recombinant rabbit antibody that recognizes HIST1H3A. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R08-9D6 is specific for human, mouse, rat HIST1H3A. The antigen is a synthetic phosphopeptide corresponding to residues surrounding thr3 of human histone h3.

Gene Expression Tested Data
Figure 1 Cerebral cortex Figure 2 Colon Figure 3 Liver Figure 4 Kidney Figure 5 Testis Figure 6 Lymph node Figure 7 RNA cell line category: Cell line enhanced (U-2 OS)
Figure 1. Recombinant Rabbit Anti-HIST1H3A (phospho Thr3) Antibody (clone R08-9D6) in WB.

Specifications

  • Immunogen
  • A synthetic phosphopeptide corresponding to residues surrounding Thr3 of human histone H3.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat HIST1H3A
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R08-9D6
  • Applications
  • Western Blot, Immunocytochemistry, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 15 kDa; Observed MW: 15 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3/A; H3FA; HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J; Histone H3.1; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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