Recombinant Rabbit Anti-HLTF Antibody (clone R03-1E4)
CAT#: VS3-FY683
This product is a recombinant rabbit antibody that recognizes HLTF. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R03-1E4 is specific for human HLTF. The antigen is human hltf synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15284/1891_F6_23_cr5bbcaa8fe600d_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49001/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows nuclear positivity of cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15284/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebellum
Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15284/37577_B_7_8.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15284/37577_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15284/37577_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000071794-HLTF
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WB
Figure 1. Recombinant Rabbit Anti-HLTF Antibody (clone R03-1E4) in WB.
Western Blot analysis of HLTF in HeLa lysates using HLTF Antibody.
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Specifications
- Immunogen
- Human HLTF Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HLTF
- Species Reactivity
- Human
- Clone
- R03-1E4
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 114 kDa; Observed MW: 114 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Helicase Like Transcription Factor; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 3; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 3; RING-Type E3 Ubiquitin Transferase HLTF; Sucrose Nonfermenting Protein 2-Like 3; RING Finger Protein 80; SMARCA3; HIP116A; SNF2L3; HIP116; RNF80
- Gene ID
- 6596
- UniProt ID
- Q14527
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HLTF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1629 | Rabbit Anti-HLTF Recombinant Antibody (clone DS1629AB) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0024-LS | Mouse Anti-HLTF Recombinant Antibody (clone HLTF8F1) | ELISA, WB, IP, IF | Humanized IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0024-LS-F(E) | Mouse Anti-HLTF Recombinant Antibody (clone HLTF8F1); Fab Fragment | ELISA, WB, IP, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0024-LS-S(P) | Mouse Anti-HLTF Recombinant Antibody (clone HLTF8F1); scFv Fragment | ELISA, WB, IP, IF | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY126 | AbPlus™ Anti-HLTF Magnetic Beads (HLTF8F1) | IP, Protein Purification |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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