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  • Recombinant Mouse Anti-HMGB1 Detection Antibody (clone 1G1)

Recombinant Mouse Anti-HMGB1 Detection Antibody (clone 1G1) (CAT#: VS3-WK1655)

This antibody is a mouse recombinant antibody against human HMGB1. The sandwich ELISA was established using matched antibody pair 1B5 and 1G1. The clone 1G1 can be used in ELISA analysis as a detection antibody at a dilution of 1/10000. It was developed by immunization of mouse with recombinant human HMGB1 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2b.


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Specific Inquiry
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
Figure 3 IHC staining of human bone marrow
Figure 3 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
Figure 4 IHC staining of human esophagus
Figure 4 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
Figure 5 IF staining of human cell line U-2 OS
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
Figure 6 IF staining of human cell line U-251 MG
Figure 6 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
Normal Tissue
Figure 7 Cerebral cortex
Figure 7 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
Figure 8 Colon
Figure 8 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
Figure 9 Liver
Figure 9 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
Figure 10 Kidney
Figure 10 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1

Specifications

  • Immunogen
  • Recombinant human HMGB1 protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b
  • Specificity
  • Human HMGB1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • 1G1
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.09% sodium azide
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • ELISA: 1/10000

Target

  • Alternative Names
  • High Mobility Group Box 1; High-Mobility Group (Nonhistone Chromosomal) Protein 1; Sulfoglucuronyl Carbohydrate Binding Protein; High Mobility Group Protein 1; Amphoterin; HMG-1
  • Long Name
  • High Mobility Group Box 1
  • Sequence Similarities
  • Belongs to the HMGB family.
  • Cellular Localization
  • Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum, Endosome, Membrane, Nucleus, Secreted
  • Post Translation Modifications
  • Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
    Acetylated on multiple sites upon stimulation with LPS.
  • Function
  • Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability

Recommended Products

Capture antibody

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "HMGB1"

Mouse Antibody

CAT Product Name Application Type
TAB-0315CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0315CL) IHC Mouse IgG
TAB-0316CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0316CL) ELISA, Inhib, FuncS, WB Mouse IgG
TAB-0317CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0317CL) ELISA, Inhib, FuncS, WB Mouse IgG
TAB-0318CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0318CL) ELISA, Inhib, FuncS, WB Mouse IgG
TAB-0315CL-F(E) Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-0315CL-F(E)) ELISA, Inhib, FuncS, WB Mouse Fab

Humanized Antibody

CAT Product Name Application Type
TAB-099CT Human Anti-HMGB1 Recombinant Antibody (TAB-099CT) IF, ELISA, IHC Human IgG1, λ
TAB-099CT-S(P) Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-099CT-S(P)) ELISA, FC, Block Human scFv
TAB-099CT-F(E) Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-099CT-F(E)) ELISA, FC, Block Humanized Fab

Human Antibody

CAT Product Name Application Type
TAB-101CT Anti-Human HMG1 Recombinant Antibody ELISA, BL Human antibody
TAB-101CT-S(P) Anti-Human HMG1 Recombinant Antibody scFv Fragment ELISA, BL Human antibody
TAB-101CT-F(E) Anti-Human HMG1 Recombinant Antibody Fab Fragment ELISA, BL Human antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1633 Rabbit Anti-HMGB1 Recombinant Antibody (clone DS1633AB) WB, IHC-P, FC Rabbit IgG
MOR-0049-FY Rabbit Anti-HMGB1 Recombinant Antibody (clone AFY0020) ICC, IHC-P, WB Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC2082 PE-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer FCM
MHC-LC2083 APC-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer FCM

Recombinant Antibody

CAT Product Name Application Type
MRO-1938-CN Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1938-CN) WB, IF, IHC, FC Rabbit IgG
MRO-1939-CN Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1939-CN) WB, IF, IHC, FC Rabbit IgG
HPAB-0101-WJ Human Anti-HMGB1 Recombinant Antibody (HPAB-0101-WJ) ELISA, WB Humanized IgG1, λ
ZG-0740J Rabbit Anti-HMGB1 Recombinant Antibody (clone 1A1) ELISA, WB, IHC Rabbit IgG
VS3-WK1654 Recombinant Mouse Anti-HMGB1 Capture Antibody (clone 1B5) ELISA Mouse IgM

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0215-FY-S(P) Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); scFv Fragment ELISA Mouse scFv
HPAB-N0137-YC-S(P) Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-N0137-YC-S(P)) ELISA Mouse scFv
HPAB-0101-WJ-S(P) Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-0101-WJ-S(P)) ELISA, WB Humanized scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0215-FY-F(E) Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); Fab Fragment ELISA Mouse Fab
HPAB-N0137-YC-F(E) Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-N0137-YC-F(E)) ELISA Mouse Fab
HPAB-0101-WJ-F(E) Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-0101-WJ-F(E)) ELISA, WB Humanized Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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