Recombinant Mouse Anti-HSP90B1 Antibody (VS-0923-FY75)
CAT#: VS-0923-FY75
The VS-0923-FY75 is a murine IgG1 antibody that binds to HSP90B1. The epitope of this antibody is within the amino acids region 501-803 of human HSP90B1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A431
Immunofluorescent staining of human cell line A431 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/14_F12_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/16_F12_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23098_B_7_5.jpg
Normal Tissue
Figure 5 Thyroid gland
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23098_B_3_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166598-HSP90B1
❮
❯
Specifications
- Immunogen
- HSP90B1 protein fragment (501-803AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HSP90B1
- Species Reactivity
- Human
- Applications
- ELISA, Western Blot
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Buffer
- Citrate Tris HCl buffer
- Preservative
- 0.02% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1/1000)
Specific conditions should be optimized by the end user.
Target
- Alternative Names
- Heat Shock Protein 90 Beta Family Member 1; Heat Shock Protein 90kDa Beta (Grp94), Member 1; Heat Shock Protein 90 KDa Beta Member 1; Tumor Rejection Antigen (Gp96) 1; 94 KDa Glucose-Regulated Protein; Tumor Rejection Antigen 1; Endoplasmin; GRP94; TRA1; Epididymis Secretory Sperm Binding Protein Li 125m; Heat Shock Protein 90kDa Beta Family Member 1
- Gene ID
- 7184
- UniProt ID
- P14625
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HSP90B1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1330-VHH | Recombinant Anti-Human HSP90B1 VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
| CAT | Product Name | Application | Type |
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| TAB-011CT-S(P) | Human Anti-HSP90B1 Recombinant Antibody; scFv Fragment (TAB-011CT-S(P)) | WB | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1691 | Rabbit Anti-HSP90B1 Recombinant Antibody (clone DS1691AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MHC-LC4334 | PE-Mamu-B*001:01/Human HSP90B1 (SDYLELDTI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0701-CN | Mouse Anti-HSP90B1 Recombinant Antibody (clone F1-D10-G3) | WB, IHC, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY235 | Rat Anti-NHP HSP90B1 Recombinant Antibody (clone SPM249) | WB, IHC, IF, FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3306 | Anti-HSP90B1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3307 | Anti-Mouse HSP90B1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3309 | Anti-Rabbit HSP90B1 Immunohistochemistry Kit | IHC |
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