Recombinant Rabbit Anti-HSPB1 Antibody (clone R03-9F4)
CAT#: VS3-FY711
This product is a recombinant rabbit antibody that recognizes HSPB1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R03-9F4 is specific for human HSPB1. The antigen is synthetic peptide of human hsp27.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47330/if_selected.jpg
Normal Tissue
Figure 2 Esophagus
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4439/17421_B_4_1_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_7_5.jpg
Normal Tissue
Figure 5 High expression in vagina
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_B_2_2.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTCEpi, MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106211-HSPB1
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WB
Figure 1. Recombinant Rabbit Anti-HSPB1 Antibody (clone R03-9F4) in WB.
Western Blot analysis of Hsp27 in K562 lysates using Hsp27 antibody.
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Specifications
- Immunogen
- Synthetic peptide of human Hsp27.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSPB1
- Species Reactivity
- Human
- Clone
- R03-9F4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 23 kDa; Observed MW: 27 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- Heat Shock Protein Family B (Small) Member 1; Estrogen-Regulated 24 KDa Protein; Stress-Responsive Protein 27; Heat Shock 27kDa Protein 1; Heat Shock 27kD Protein 1; Heat Shock 27 KDa Protein; 28 KDa Heat Shock Protein; HSP27; HSP28; SRP27
- Gene ID
- 3315
- UniProt ID
- P04792
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HSPB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1699 | Rabbit Anti-HSPB1 Recombinant Antibody (clone DS1699AB) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2094 | PE-B*07:02/Human HSPB1 (APAYSRAL) MHC Tetramer | FCM | |
| MHC-LC2095 | APC-B*07:02/Human HSPB1 (APAYSRAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1942-CN | Rabbit Anti-HSPB1 Polyclonal Antibody (MRO-1942-CN) | WB, IF, IHC | Rabbit IgG |
| FAMAB-1155CQ | Mouse Anti-HSPB1 Recombinant Antibody (clone SAIC-23E-30) | Immuno-MRM, ELISA | Mouse IgG1 |
| FAMAB-1156CQ | Mouse Anti-HSPB1 Recombinant Antibody (clone SAIC-23B-22) | Immuno-MRM, ELISA | Mouse IgG1 |
| FAMAB-1157CQ | Mouse Anti-HSPB1 Recombinant Antibody (clone SAIC-23C-35) | Immuno-MRM, WB | Mouse IgG1 |
| VS3-QX564 | Mouse Anti-HSPB1 Recombinant Antibody (clone 9G1-4D1-2G2) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1360 | AbPlus™ Anti-HSPB1 Magnetic Beads (VS-0724-YC1360) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1076 | Anti-HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3335 | Anti-Mouse HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3334 | Anti-Human HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3336 | Anti-Chicken HSPB1 Immunohistochemistry Kit | IHC |
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