Recombinant Rabbit Anti-IL12B Capture Antibody (clone R07-6Y2) (CAT#: VS3-WK1712)
This antibody is a rabbit recombinant antibody against human IL12B. It was developed by immunization of rabbit with recombinant human IL12B protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R07-6Y2 and R06-2G1. The clone R07-6Y2 can be used as a capture antibody in ELISA analysis to identify human IL12B.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in a subset of lymphoid cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40970/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in a subset of lymphoid cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40970/ihc_selected.jpg
Figure 2 Cerebral cortex
(Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40970/86793_B_7_5.jpg
(Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40970/86793_B_7_5.jpg
Figure 3 Colon
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40970/86793_A_7_3.jpg
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40970/86793_A_7_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40970/86793_A_7_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40970/86793_A_7_4.jpg
Figure 5 RNA cell line category: Cell line enhanced (SCLC-21H)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113302-IL12B
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113302-IL12B
Specifications
- Immunogen
- Recombinant human IL12B protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL12B
- Species Reactivity
- Human
- Clone
- R07-6Y2
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Interleukin 12B
- Gene ID
- 3593
- UniProt ID
- P29460
- Long Name
- Interleukin 12B
- Sequence Similarities
- Belongs to the IL-12B family.Curated
- Cellular Localization
- Secreted
- Post Translation Modifications
- Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
- Protein Refseq
- NP_002178.2
- Function
- Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0287MZ | Chicken Anti-Interleukin-12β Polyclonal IgY | WB | Chicken antibody |
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