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    Recombinant Rabbit Anti-Itgal Antibody (clone R08-3I4)

    CAT#: VS3-FY771

    This product is a recombinant rabbit antibody that recognizes Itgal. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R08-3I4 is specific for human, mouse Itgal. The antigen is mouse cd11a recombinant protein.

    Gene Expression Tested Data
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    Figure 1 Bone marrow Figure 2 Lymph node Figure 3 RNA cell line category: Cell line enriched (HMC-1)
    ❯
    ❮
    Figure 1. Recombinant Rabbit Anti-Itgal Antibody (clone R08-3I4) in IHC-P.
    ❯

    Specifications

    • Immunogen
    • mouse CD11a recombinant protein.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse Itgal
    • Species Reactivity
    • Human, Mouse
    • Clone
    • R08-3I4
    • Conjugate
    • Unconjugated
    • MW
    • Calculated MW: 128 kDa; Observed MW: 160 kDa

    Product Property

    • Purification
    • Affinity Purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Buffer
    • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
    • Preservative
    • 0.01% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000
      Immunohistochemistry-Paraffin: 1/50-1/100

    Target

    • Alternative Names
    • Integrin Subunit Alpha L
    • Sequence Similarities
    • Belongs to the integrin alpha chain family.
    • Cellular Localization
    • Cell membrane, Membrane
    • Post Translation Modifications
    • In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents.
    • Function
    • Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R (PubMed:11812992, PubMed:15528364).
      Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055).
      Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity (PubMed:15356110).
      Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992).
      Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (By similarity).
      Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).
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    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Datasheet

    Datasheet - VS3-FY771.pdf

    MSDS

    MSDS - VS3-FY771.pdf

    COA

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

    Lot Number:

    Secondary Antibody

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    • Product Name

    Isotype Control

    • CAT
    • Product Name

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