Recombinant Rabbit Anti-KDM4A Antibody (clone R01-7F2) (CAT#: VS3-FY791)
This product is a recombinant rabbit antibody that recognizes KDM4A. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R01-7F2 is specific for human KDM4A. The antigen is synthetic peptides derived from human kdm4a / jhdm3a / jmjd2a.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/ihc_selected.jpg
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/75_D3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/75_D3_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/74_D3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/74_D3_1_blue_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_B_7_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_B_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_A_4_6.jpg
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_A_4_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000066135-KDM4A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000066135-KDM4A
Specifications
- Immunogen
- Synthetic peptides derived from human KDM4A / JHDM3A / JMJD2A.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KDM4A
- Species Reactivity
- Human
- Clone
- R01-7F2
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 121 kDa; Observed MW: 150 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Lysine Demethylase 4A; JmjC Domain-Containing Histone Demethylation Protein 3A; Jumonji C Domain-Containing Histone Demethylase 3A; Jumonji Domain-Containing Protein 2A; Lysine (K)-Specific Demethylase 4A; Jumonji Domain Containing 2A; Tudor Domain Containing 14A; Jumonji Domain Containing 2; JMJD2A
- Gene ID
- 9682
- UniProt ID
- O75164
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-366 | Recombinant Human Anti-Human KDM4A Antibody Fab Fragment, clone D266 | IP, IF, ELISA, IP-MS | Fab |
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For Research Use Only. Not For Clinical Use.
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