Recombinant Rabbit Anti-KDM4A Antibody (clone R01-7F2) (CAT#: VS3-FY791)
This product is a recombinant rabbit antibody that recognizes KDM4A. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R01-7F2 is specific for human KDM4A. The antigen is synthetic peptides derived from human kdm4a / jhdm3a / jmjd2a.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/ihc_selected.jpg
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/75_D3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/75_D3_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/74_D3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/74_D3_1_blue_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_B_7_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_B_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_A_4_6.jpg
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_A_4_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000066135-KDM4A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000066135-KDM4A
Specifications
- Immunogen
- Synthetic peptides derived from human KDM4A / JHDM3A / JMJD2A.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KDM4A
- Species Reactivity
- Human
- Clone
- R01-7F2
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 121 kDa; Observed MW: 150 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Lysine Demethylase 4A; JmjC Domain-Containing Histone Demethylation Protein 3A; Jumonji C Domain-Containing Histone Demethylase 3A; Jumonji Domain-Containing Protein 2A; Lysine (K)-Specific Demethylase 4A; Jumonji Domain Containing 2A; Tudor Domain Containing 14A; Jumonji Domain Containing 2; JMJD2A
- Gene ID
- 9682
- UniProt ID
- O75164
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-366 | Recombinant Human Anti-Human KDM4A Antibody Fab Fragment, clone D266 | IP, IF, ELISA, IP-MS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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