Recombinant Rat Anti-KITLG Detection Antibody (clone 1D11) (CAT#: VS3-WK1969)
This antibody is a rat recombinant antibody against human KITLG. It was developed by immunization of rat with recombinant human KITLG protein and then was produced as a recombinant antibody. The isotype of this product is rat IgG2b. 17E8 and 1D11 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone 1D11 can be used in ELISA analysis to identify human KITLG.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/1515_E4_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/1515_E4_1_selected.jpg
Figure 2 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/159850_B_5_4_selected.jpg
(Immunohistochemical staining of human bone marrow shows nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/159850_B_5_4_selected.jpg
Figure 3 Spleen
(Cells in red pulp
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/159850_B_7_4.jpg
(Cells in red pulp
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/159850_B_7_4.jpg
Figure 4 RNA cell line category: Cell line enhanced (CACO-2, SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000049130-KITLG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000049130-KITLG
Specifications
- Immunogen
- Recombinant human KITLG protein
- Host Species
- Rat
- Type
- Rat IgG2b
- Specificity
- Human KITLG
- Species Reactivity
- Human
- Clone
- 1D11
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- KIT Ligand; Mast Cell Growth Factor; Stem Cell Factor; Familial Progressive Hyperpigmentation 2; Steel Factor; C-Kit Ligand; MGF; SCF; DFNA69
- Gene ID
- 4254
- UniProt ID
- P21583
- Long Name
- KIT Ligand
- Sequence Similarities
- Belongs to the SCF family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Secreted
- Post Translation Modifications
- A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain.
- Protein Refseq
- NP_000890.1
- Function
- Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-601 | Recombinant Anti-human KITLG Antibody | WB, IF, FuncS | IgG |
| MHH-601 | Recombinant Human Anti-human KITLG Antibody | ELISA, WB, IF, FuncS | IgG |
| MOB-1398CT | Recombinant Mouse anti-Human KITLG Monoclonal antibody (2.3_3I6-2D20) | ELISA | |
| MRO-1369-CN | Recombinant Rabbit Anti-KITLG Monoclonal Antibody (CBACN-497) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ779 | Rabbit Anti-KITLG Recombinant Antibody (VS3-CJ779) | WB, ICC, IF, IHC, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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