Recombinant Rabbit Anti-Klf4 Antibody (clone R03-2I8)
CAT#: VS3-FY798
This product is a recombinant rabbit antibody that recognizes Klf4. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R03-2I8 is specific for human, mouse, Hamster Klf4. The antigen is mouse klf4 recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
Normal Tissue
Figure 2 High expression in esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, hTCEpi, hTERT-HME1, OE19, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
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IHC-P
Figure 1. Recombinant Rabbit Anti-Klf4 Antibody (clone R03-2I8) in IHC-P.
Immunohistochemical analysis of paraffin-embedded mouse colon using an antibody to KLF4. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Mouse KLF4 Recombinant Protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Hamster Klf4
- Species Reactivity
- Human, Mouse, Hamster
- Clone
- R03-2I8
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 52 kDa; Observed MW: 52 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Kruppel Like Factor 4; Epithelial Zinc Finger Protein EZF; Gut-Enriched Krueppel-Like Factor; Kruppel-Like Factor 4 (Gut); Gut Kruppel-Like Factor; GKLF; EZF; Endothelial Kruppel-Like Zinc Finger Protein; Krueppel-Like Factor 4
- Gene ID
- 9314
- UniProt ID
- O43474
- Cellular Localization
- Nucleus
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KLF4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1026CT | Recombinant Mouse anti-Human KLF4 Monoclonal antibody (2F6) | ELISA, ICC, IF, IHC-P, WB | |
| ZG-0387J | Mouse Anti-KLF4 Recombinant Antibody (ZG-0387J) | WB, IHC, ELISA | Mouse IgG |
| ZG-0388J | Mouse Anti-KLF4 Recombinant Antibody (ZG-0388J) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0786J | Rabbit Anti-KLF4 Recombinant Antibody (clone 3A1) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS3-XY980 | Mouse Anti-KLF4 Recombinant Antibody (clone 1E5) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1956 | Hi-Affi™ Recombinant Rabbit Anti-KLF4 Monoclonal Antibody (DS1956AB) | ELISA, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC96 | AbPlus™ Anti-KLF4 Magnetic Beads (VS-0724-YC96) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC36 | Mouse Anti-KLF4 Recombinant Antibody (VS-0924-YC36) - Cancer Stem Cell Marker | IHC, WB, IF | |
| VS-0924-YC63 | Rabbit Anti-KLF4 Antibody (VS-0924-YC63) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3795 | Anti-KLF4 Immunohistochemistry Kit | IHC |
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