Recombinant Mouse Anti-KRT20 Antibody (clone 10B5-4H7-5A9)
CAT#: VS3-FY825
This product is a recombinant mouse antibody that recognizes KRT20. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 10B5-4H7-5A9 is specific for human KRT20. The antigen is synthetic peptide derived from human cytokeratin 20.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24684/if_selected.jpg
Normal Tissue
Figure 2 High expression in small intestine
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24309/54018_A_5_2_val_selected.jpg
Normal Tissue
Figure 3 Low expression in liver
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24309/54018_A_7_4_val_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32/127_A_8_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171431-KRT20
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IHC-P
Figure 1. Recombinant Mouse Anti-KRT20 Antibody (clone 10B5-4H7-5A9) in IHC-P.
Immunohistochemical analysis of paraffin-embedded colon carcinoma using cytokeratin 20 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human cytokeratin 20.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human KRT20
- Species Reactivity
- Human
- Clone
- 10B5-4H7-5A9
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Keratin 20; Keratin 20, Type I; Protein IT; CK-20; K20; Keratin, Type I Cytoskeletal 20; Cytokeratin 20
- Gene ID
- 54474
- UniProt ID
- P35900
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin
- Post Translation Modifications
- Hyperphosphorylation at Ser-13 oCcurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
- Protein Refseq
- NP_061883.1
- Function
- Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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Isotype Control
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Reconstitution Buffer
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Recommended Dilution Buffer
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See other products for "KRT20"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2245MZ | Recombinant Mouse Anti-Human KRT20 Antibody (clone Lt30.9) | FC, IHC-P | Mouse antibody |
| MRO-0444-CN | Recombinant Rabbit Anti-KRT20 Monoclonal Antibody (CBACN-182) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-1052F | Mouse Anti-KRT20 Recombinant Antibody (clone MKs20-8) | IHC-Fr, IHC-P, IP, WB, ICC | Mouse IgG2a |
| ZG-0325U | Rabbit Anti-KRT20 Recombinant Antibody (clone E19-I) | IHC-P | Rabbit IgG |
| VS11-QX93 | Mouse Anti-KRT20 Recombinant Antibody (clone 10A2) | ELISA, WB, IHC | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1985 | Hi-Affi™ Recombinant Rabbit Anti-KRT20 Monoclonal Antibody (DS1985AB) | IHC-Fr, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1216 | Anti-KRT20 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3867 | Anti-Rat KRT20 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3866 | Anti-Human KRT20 Immunohistochemistry Kit | IHC |
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