Recombinant Mouse Anti-KRT20 Antibody (clone 10B5-4H7-5A9) (CAT#: VS3-FY825)
This product is a recombinant mouse antibody that recognizes KRT20. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 10B5-4H7-5A9 is specific for human KRT20. The antigen is synthetic peptide derived from human cytokeratin 20.
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IHC-P
Figure 1. Recombinant Mouse Anti-KRT20 Antibody (clone 10B5-4H7-5A9) in IHC-P.
Immunohistochemical analysis of paraffin-embedded colon carcinoma using cytokeratin 20 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide derived from human cytokeratin 20.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human KRT20
- Species Reactivity
- Human
- Clone
- 10B5-4H7-5A9
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Keratin 20; Keratin 20, Type I; Protein IT; CK-20; K20; Keratin, Type I Cytoskeletal 20; Cytokeratin 20
- Gene ID
- 54474
- UniProt ID
- P35900
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin
- Post Translation Modifications
- Hyperphosphorylation at Ser-13 oCcurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
- Protein Refseq
- NP_061883.1
- Function
- Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1373z | Mouse Anti-KRT20 Recombinant Antibody (clone 27D4) | WB, ICC, IF, FC, ELISA | Mouse IgG2a |
| MOB-2245MZ | Recombinant Mouse Anti-Human KRT20 Antibody (clone Lt30.9) | FC, IHC-P | Mouse antibody |
| ZG-0248J | Mouse Anti-KRT20 Recombinant Antibody (ZG-0248J) | IHC-P, IF | Mouse IgG |
| ZG-1052F | Mouse Anti-KRT20 Recombinant Antibody (clone MKs20-8) | IHC-Fr, IHC-P, IP, WB, ICC | Mouse IgG2a |
| VS7-0425-WR264 | Mouse Anti-KRT20 Recombinant Antibody (clone 2C7B9) | IHC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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