Recombinant Mouse Anti-KRT5 Antibody (clone 6G9-7H1-5E2)
CAT#: VS3-FY827
This product is a recombinant mouse antibody that recognizes KRT5. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 6G9-7H1-5E2 is specific for human KRT5. The antigen is synthetic peptide derived from human cytokeratin 5.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line hTCEpi
Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HaCaT, HBEC3-KT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
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IHC-P
Figure 1. Recombinant Mouse Anti-KRT5 Antibody (clone 6G9-7H1-5E2) in IHC-P.
Immunohistochemical analysis of paraffin-embedded squamous cell lung carcinoma using cytokeratin 5 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human cytokeratin 5.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human KRT5
- Species Reactivity
- Human
- Clone
- 6G9-7H1-5E2
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Keratin 5; Keratin 5 (Epidermolysis Bullosa Simplex, Dowling-Meara/Kobner/Weber-Cockayne Types); Epidermolysis Bullosa Simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; Type-II Keratin Kb5; Keratin 5, Type II; Cytokeratin-5; CK-5; K5; Keratin, Type II Cytoskeletal 5
- Gene ID
- 3852
- UniProt ID
- P13647
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filament, Keratin
- Post Translation Modifications
- Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle (By similarity).
Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis (By similarity).
CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury (By similarity).
- Protein Refseq
- NP_000415.2
- Function
- Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KRT5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1510MZ | Recombinant Mouse Anti-Human KRT5 Antibody (clone YN37) | ICC, IHC-Fr, IHC-P | Mouse antibody |
| ZG-0250J | Mouse Anti-KRT5 Recombinant Antibody (ZG-0250J) | IHC-P, IF | Mouse IgG |
| ZG-0251J | Mouse Anti-KRT5 Recombinant Antibody (ZG-0251J) | IHC-P, IF | Mouse IgG |
| VS7-0425-WR265 | Mouse Anti-KRT5 Monoclonal Antibody (VS7-0425-WR265) | IHC-P, IF, ICC, ELISA | Mouse IgG |
| VS7-0425-WR266 | Mouse Anti-KRT5 Monoclonal Antibody (VS7-0425-WR266) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1990 | Hi-Affi™ Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (DS1990AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1518 | AbPlus™ Anti-KRT5 Magnetic Beads (VS-0724-YC1518) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC636 | CytoStream™ Rabbit Anti-KRT5 Recombinant Antibody (VS13-YC636) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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