Recombinant Mouse Anti-KRT5 Antibody (VS4-CJ8)
CAT#: VS4-CJ8
This mouse anti-KRT5 antibody is a product that recognizes human KRT5. The immunogen of this antibody is a synthesized peptide derived from human Cytokeratin 5. The mAb has been tested for use in ELISA, Immunohistochemistry (1:20-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line hTCEpi
Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HaCaT, HBEC3-KT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
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Specifications
- Immunogen
- Synthesized peptide derived from human Cytokeratin 5
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Human KRT5
- Species Reactivity
- Human
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:20-1:200).
Target
- Alternative Names
- K5; CK5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; KRT5A
- Gene ID
- 3852
- UniProt ID
- P13647
- Long Name
- Keratin 5
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filament, Keratin
- Post Translation Modifications
- Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle (By similarity).
Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis (By similarity).
CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury (By similarity).
- Protein Refseq
- NP_000415.2
- Function
- Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KRT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1990 | Hi-Affi™ Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (DS1990AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0459F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0459F) | IHC, IF, ELISA | Mouse IgG |
| ZG-0460F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0460F) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0467F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0467F) | WB, ELISA | Mouse IgG |
| ZG-0468F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0468F) | WB, IHC, ELISA | Mouse IgG |
| VS3-XY997 | Mouse Anti-KRT5 Recombinant Antibody (clone 7H8C4) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1518 | AbPlus™ Anti-KRT5 Magnetic Beads (VS-0724-YC1518) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC636 | CytoStream™ Rabbit Anti-KRT5 Recombinant Antibody (VS13-YC636) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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