Recombinant Rabbit Anti-LEF1 Antibody (clone R02-3A4) (CAT#: VS3-FY845)
This product is a recombinant rabbit antibody that recognizes LEF1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R02-3A4 is specific for human LEF1. The antigen is synthetic peptide of human lef1.
Specific Inquiry
ICC
Figure 1. Recombinant Rabbit Anti-LEF1 Antibody (clone R02-3A4) in ICC.
Immunocytochemical analysis of LEF1 (green) in Jurkat using LEF1 antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-LEF1 Antibody (clone R02-3A4) in WB.
Western Blot analysis of LEF1 in Jurkat lysates using LEF1 antibody.
Specifications
- Immunogen
- Synthetic peptide of human LEF1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LEF1
- Species Reactivity
- Human
- Clone
- R02-3A4
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 44 kDa; Observed MW: 25-58 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- LEF1; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha
- Gene ID
- 51176
- UniProt ID
- Q9UJU2
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "LEF1"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4028z | Mouse Anti-LEF1 Recombinant Antibody (clone 14F1) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MOB-1081CT | Recombinant Mouse anti-Human LEF1 Monoclonal antibody (2D4) | WB | |
| ZG-0894F | Mouse Anti-LEF1 Recombinant Antibody (clone REMB1) | WB, IF, IHC-P | Mouse IgG1 |
| ZG-0896F | Mouse Anti-LEF1 Recombinant Antibody (clone REMB6) | WB, IHC | Mouse IgG1 |
| VS3-WK1203 | Mouse Anti-LEF1 Recombinant Antibody (VS3-WK1203) | WB | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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