Recombinant Mouse Anti-LGALS3 Antibody (VS-0923-FY63) (CAT#: VS-0923-FY63)
The VS-0923-FY63 is a murine IgG1 antibody that binds to LGALS3. This antibody was originally generated in mice immuned with the full length of human LGALS3.
Specific Inquiry
Custom Production
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76528/2057_C12_5_selected.jpg
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76528/2057_C12_5_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5191/639_D6_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5191/639_D6_1_selected.jpg
Figure 3 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5191/ihc_selected.jpg
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5191/ihc_selected.jpg
Figure 4 IHC staining of human cerebral cortex
(Low expression. RNA expression: 148.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_B_9_5_val_selected.jpg
(Low expression. RNA expression: 148.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_B_9_5_val_selected.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_9_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_9_4.jpg
Figure 6 Kidney
(Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_8_5.jpg
(Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_8_5.jpg
Figure 7 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_6_6.jpg
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_6_6.jpg
Figure 8 Skin
(Eccrine glands
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Lymphocytes
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_B_7_1.jpg
(Eccrine glands
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Lymphocytes
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_B_7_1.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_7_8.jpg
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_7_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, CAPAN-2, OE19, SK-MEL-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131981-LGALS3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131981-LGALS3
Specifications
- Immunogen
- The full-length Galectin-3 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LGALS3
- Species Reactivity
- Human
- Applications
- ELISA, Western Blot
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Buffer
- Citrate Tris HCl buffer
- Preservative
- 0.02% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1/1000)
Specific conditions should be optimized by the end user.
Target
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1583z | Mouse Anti-LGALS3 Recombinant Antibody (clone 32D7) | WB, ELISA, FC, IHC, FuncS | Mouse IgG1 |
| MOB-244YJL | Rabbit Anti-LGALS3 Recombinant Antibody (clone CBL228) | WB, ELISA, FC, ICC, IF, IP | Rabbit IgG |
| MOB-245YJL | Mouse Anti-LGALS3 Recombinant Antibody (clone CBL229) | WB, ELISA, IP | Mouse IgG1 |
| VS3-WK1244 | Mouse Anti-LGALS3 Recombinant Antibody (VS3-WK1244) | WB, IHC-P, ELISA | Mouse IgG |
| VS-0824-YJ35 | Human Anti-LGALS3 Recombinant Antibody (clone M3/38) | ELISA, WB, IHC, FC | Chimeric (rat/human) IgG1 kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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