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    Recombinant Mouse Anti-LMNB1 Antibody (VS4-CJ18) (CAT#: VS4-CJ18)

    This mouse anti-LMNB1 antibody is a product that recognizes human, mouse, rat LMNB1. The immunogen of this antibody is a recombinant protein corresponding to LMNB1. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunoprecipitation (1:200-1:2000).

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    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line MCF7
    Figure 1 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/if_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/950_C1_1_selected.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79757/1894_G3_4_selected.jpg
    Figure 4 IHC staining of human colon
    Figure 4 IHC staining of human colon
    (Immunohistochemical staining of human colon shows nuclear membrane positivity in glandular and stroma cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/ihc_selected.jpg
    Figure 5 IHC staining of human stomach
    Figure 5 IHC staining of human stomach
    (Immunohistochemical staining of human stomach shows strong nuclear membranous positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/155655_A_3_1_selected.jpg
    Figure 6 IHC staining of human esophagus
    Figure 6 IHC staining of human esophagus
    (Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79954/171056_B_6_1_selected.jpg
    Normal Tissue
    Figure 7 Cerebral cortex
    Figure 7 Cerebral cortex
    (Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_B_8_5.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_7_4.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Leydig cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_5_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_8_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113368-LMNB1

    Specifications

    • Immunogen
    • Recombinant Protein
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human, Mouse, Rat LMNB1
    • Species Reactivity
    • Human, Mouse, Rat
    • Applications
    • ELISA, WB, IP
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Immunogen affinity chromatography
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • 50% Glycerol, PBS, pH 7.4.
    • Preservative
    • 0.02% Sodium Azide
    • Storage
    • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

    Applications

    • Application Notes
    • This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunoprecipitation (1:200-1:2000).

    Target

    • Alternative Names
    • LMN; ADLD; LMN2; LMNB; MCPH26
    • Long Name
    • Lamin B1
    • Sequence Similarities
    • Belongs to the intermediate filament family.
    • Cellular Localization
    • Intermediate filament, Nucleus
    • Post Translation Modifications
    • B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
    • Function
    • Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    MOB-2469CT Recombinant Mouse anti-Chicken LMNB1 Monoclonal antibody (T70) WB
    MRO-0913-CN Recombinant Rabbit Anti-LMNB1 Monoclonal Antibody (CBACN-342) WB, IF, IHC, IP Rabbit IgG
    MRO-0914-CN Recombinant Rabbit Anti-LMNB1 Monoclonal Antibody (JM099-03) WB, IHC, FC Rabbit IgG
    VS3-QX694 Mouse Anti-LMNB1 Recombinant Antibody (clone 5G8-D3-H7) WB Mouse IgG2b
    VS3-QX1150 Mouse Anti-LMNB1 Recombinant Antibody (clone 7B7) WB Mouse IgG1

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