Recombinant Rabbit Anti-MAPT (phospho Thr181) Antibody (clone R06-7C6)
CAT#: VS3-FY897
This product is a recombinant rabbit antibody that recognizes MAPT. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R06-7C6 is specific for human MAPT. The antigen is a synthetic phosphopeptide corresponding to residues surrounding thr181 of human tau.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48895/1070_E6_2_selected.jpg
❮
❯
WB
Figure 1. Recombinant Rabbit Anti-MAPT (phospho Thr181) Antibody (clone R06-7C6) in WB.
Western Blot analysis of Phospho-Tau (Thr181) in human fetal brain lysates using Phospho-Tau (Thr181) Antibody.
❮
❯
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Thr181 of human Tau.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAPT
- Species Reactivity
- Human
- Clone
- R06-7C6
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 79 kDa; Observed MW: 50-80 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Microtubule Associated Protein Tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; Protein Phosphatase 1, Regulatory Subunit 103; Neurofibrillary Tangle Protein; Paired Helical Filament-Tau; PHF-Tau; MAPTL; MTBT1; TAU
- Gene ID
- 4137
- UniProt ID
- P10636
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Secreted
- Post Translation Modifications
- Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK1, CDK1, CDK5, GSK3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in the form associated with paired helical filaments (PHF-tau)), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1, MARK2, MARK3 or MARK4), causing detachment from microtubules, and their disassembly (PubMed:7706316, PubMed:23666762).
Phosphorylation decreases with age. Phosphorylation within tau/MAP's repeat domain or in flanking regions seems to reduce tau/MAP's interaction with, respectively, microtubules or plasma membrane components (PubMed:7706316).
Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis. Phosphorylation at Ser-548 by GSK3B reduces ability to bind and stabilize microtubules. Phosphorylation at Ser-579 by BRSK1 and BRSK2 in neurons affects ability to bind microtubules and plays a role in neuron polarization. Phosphorylated at Ser-554, Ser-579, Ser-602, Ser-606 and Ser-669 by PHK. Phosphorylation at Ser-214 by SGK1 mediates microtubule depolymerization and neurite formation in hippocampal neurons. There is a reciprocal down-regulation of phosphorylation and O-GlcNAcylation. Phosphorylation on Ser-717 completely abolishes the O-GlcNAcylation on this site, while phosphorylation on Ser-713 and Ser-721 reduces glycosylation by a factor of 2 and 4 respectively. Phosphorylation on Ser-721 is reduced by about 41.5% by GlcNAcylation on Ser-717. Dephosphorylated at several serine and threonine residues by the serine/threonine phosphatase PPP5C.
Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity).
PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
O-glycosylated. O-GlcNAcylation content is around 8.2%. There is reciprocal down-regulation of phosphorylation and O-GlcNAcylation. Phosphorylation on Ser-717 completely abolishes the O-GlcNAcylation on this site, while phosphorylation on Ser-713 and Ser-721 reduces O-GlcNAcylation by a factor of 2 and 4 respectively. O-GlcNAcylation on Ser-717 decreases the phosphorylation on Ser-721 by about 41.5%.
Glycation of PHF-tau, but not normal brain TAU/MAPT. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
- Protein Refseq
- NP_001116538.2; NP_001116539.1; NP_001190181.1
- Function
- Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311).
The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270).
Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY897. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MAPT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-305 | Chicken Anti-MAPT Recombinant Antibody; scFv Fragment | WB, ELISA, FuncS | Chicken scFv |
| PSBL-340 | Mouse Anti-MAPT Recombinant Antibody (clone AT8); scFv Fragment | ELISA, FuncS | Mouse scFv |
| PSBL-674 | Chicken Anti-MAPT Recombinant Antibody (clone pT231/pS235_1); scFv Fragment | WB, ELISA, FACS, Block | Chicken scFv |
| PSBL-705 | Mouse Anti-MAPT Recombinant Antibody (clone DC8E8); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBL-706 | Rabbit Anti-MAPT Recombinant Antibody scFv Fragment (PSBL-706) | WB, IF, FuncS | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0733MZ | Chicken Anti-TAU Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-174 | Recombinant Mouse Anti-Tau5 Antibody | WB, ELISA, Neut, FuncS | IgG |
| PABX-174-S (P) | Recombinant Mouse Anti-Tau5 Antibody scFv Fragment | WB, ELISA, Neut, FuncS | scFv |
| PABX-174-F (E) | Recombinant Mouse Anti-Tau5 Antibody Fab Fragment | WB, ELISA, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2164 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (DS2164AB) | IHC-P | IgG |
| MOR-4513 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH21DS) | WB, IHC-P, FC | IgG |
| MOR-4714 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH228DS) | WB, IF, ICC, IHC-P, FC | IgG |
| MOR-4715 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH229DS) | WB, IF, ICC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0196-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0196-YJ-F(E)) | ELISA | Mouse Fab |
| HPAB-0197-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0197-YJ-F(E)) | ELISA | Mouse Fab |
| HPAB-0198-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody (clone 13A6); Fab Fragment | IA, IP | Mouse Fab |
| HPAB-0199-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody (clone 10H8); Fab Fragment | ELISA, Quantification | Mouse Fab |
| HPAB-0200-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody (clone 19G10); Fab Fragment | ELISA, Quantification | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0484-FY | Mouse Anti-MAPT Recombinant Antibody (HPAB-0484-FY) | ELISA, Cyt, WB, IP | Mouse IgG |
| HPAB-0485-FY | Mouse Anti-MAPT Recombinant Antibody (HPAB-0485-FY) | WB, IP, ELISA, IF, FC | Mouse IgG |
| VS3-FY894 | Recombinant Rabbit Anti-MAPT (phospho Ser202) Antibody (clone R01-5A7) | WB, IP | Rabbit IgG |
| VS3-FY895 | Recombinant Rabbit Anti-MAPT Antibody (clone R03-9I2) | WB | Rabbit IgG |
| VS3-FY896 | Recombinant Rabbit Anti-MAPT Antibody (clone R04-2G8) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY182 | AbPlus™ Anti-MAPT Magnetic Beads (VS-0424-XY182) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY121 | Human Anti-MAPT (clone IPN002) scFv-Fc Chimera | ELISA, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4255 | Anti-MAPT Immunohistochemistry Kit | IHC | |
| VS-0525-XY4256 | Anti-Mouse MAPT Immunohistochemistry Kit | IHC | |
| VS-0525-XY4257 | Anti-Rat MAPT Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC120 | Recombinant Anti-MAPT (AA 220-224 x AA 506-511) Biparatopic Antibody, Tandem scFv (Clone 13A6 x Clone 19G10) | ELISA | Tandem scFv |
| VS-0525-YC121 | Recombinant Anti-MAPT (AA 506-511 x AA 537-541) Biparatopic Antibody, Tandem scFv (Clone 19G10 x Clone 10H8) | ELISA | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC216 | Recombinant Anti-MAPT Eliminating Antibody, pH-Sensitive (VS-0625-YC216) | Antigen-Sweeping In Vivo. | Mouse IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
-2.png)
Application: ELISA, WB
Application: FC, IA, IF, IP, IHC, FuncS
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, FuncS
Application: ELISA, Neut
Application: ELISA, IHC, IF, IP, FC, FuncS
Application: FC, IA, IF, IP, IHC, FuncS
Application: ELISA, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
