Recombinant Mouse Anti-MERTK Antibody (clone 16D1) (CAT#: VS4-CJ33)

• Immunogen
• Recombinant MERTK protein (21aa-505aa)

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human MERTK

• Species Reactivity
• Human

• Clone
• 16D1

• Applications
• ELISA, WB, IF, FC

• Conjugate
• Unconjugated

• Purification
• Protein A purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, PBS, pH 7.3.

• Preservative
• 0.1% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

• Alternative Names
• MER; RP38; c-Eyk; c-mer; Tyro12

• Gene ID
• 10461

• UniProt ID
• Q12866

• Long Name
• MER proto-oncogene, tyrosine kinase

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

• Cellular Localization
• Cell membrane, Membrane

• Post Translation Modifications
• Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity).

• Protein Refseq
• NP_006334.2

• Function
• Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed:32640697).
  Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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