Recombinant Mouse Anti-MERTK Antibody (clone 16D1) (CAT#: VS4-CJ33)
This mouse anti-MERTK antibody (clone 16D1) is a product that recognizes human MERTK. The immunogen of this antibody is Recombinant MERTK protein (21aa-505aa). This product was purified by protein A purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75622/1740_F11_62_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75622/1740_F11_62_selected.jpg
Figure 2 Adrenal gland
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36196/75498_B_6_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36196/75498_B_6_5.jpg
Figure 3 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36196/75498_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36196/75498_A_9_4.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36196/75498_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36196/75498_A_8_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36196/75498_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36196/75498_A_4_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (CACO-2, Hep G2, HUVEC TERT2, RT4)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153208-MERTK
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153208-MERTK
Specifications
- Immunogen
- Recombinant MERTK protein (21aa-505aa)
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human MERTK
- Species Reactivity
- Human
- Clone
- 16D1
- Applications
- ELISA, WB, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.3.
- Preservative
- 0.1% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Target
- Alternative Names
- MER; RP38; c-Eyk; c-mer; Tyro12
- Gene ID
- 10461
- UniProt ID
- Q12866
- Long Name
- MER proto-oncogene, tyrosine kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity).
- Protein Refseq
- NP_006334.2
- Function
- Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed:32640697).
Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "MERTK"
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1725CQ | Recombinant Mouse Anti-MERTK Antibody (Y323) | BL, WB, IP, IHC-P | IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2214 | Hi-Affi™ Recombinant Rabbit Anti-MERTK Monoclonal Antibody (DS2214AB) | WB, IHC, IP, FC | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-CN | Mouse Anti-MERTK Recombinant Antibody (HPAB-0215-CN) | WB, ELISA | Mouse IgG |
| HPAB-0216-CN | Mouse Anti-MERTK Recombinant Antibody (HPAB-0216-CN) | WB, ELISA | Mouse IgG |
| FN-187CQ | Recombinant Rat Anti-MERTK Antibody (CBL-1327) | FC, FuncS, IF, IHC-Fr, WB, IP | Rat IgG2a, κ |
| ZG-0138U | Human Anti-MERTK Recombinant Antibody (clone 8H11) | ELISA | Humanized IgG4 |
| VS3-FY923 | Recombinant Rabbit Anti-MERTK Antibody (clone R07-6H2) | WB, IHC-P, IP | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-CN-S(P) | Mouse Anti-MERTK Recombinant Antibody; scFv Fragment (HPAB-0215-CN-S(P)) | WB, ELISA | Mouse scFv |
| HPAB-0216-CN-S(P) | Mouse Anti-MERTK Recombinant Antibody; scFv Fragment (HPAB-0216-CN-S(P)) | WB, ELISA | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-CN-F(E) | Mouse Anti-MERTK Recombinant Antibody; Fab Fragment (HPAB-0215-CN-F(E)) | WB, ELISA | Mouse Fab |
| HPAB-0216-CN-F(E) | Mouse Anti-MERTK Recombinant Antibody; Fab Fragment (HPAB-0216-CN-F(E)) | WB, ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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