Recombinant Rabbit Anti-MGMT Antibody (clone R01-8B1) (CAT#: VS3-FY926)
This product is a recombinant rabbit antibody that recognizes MGMT. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R01-8B1 is specific for human MGMT. The antigen is human mgmt synthetic peptide.
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WB
Figure 1. Recombinant Rabbit Anti-MGMT Antibody (clone R01-8B1) in WB.
Western Blot analysis of MGMT in Jurkat lysates using MGMT antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-MGMT Antibody (clone R01-8B1) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MGMT antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Human MGMT Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MGMT
- Species Reactivity
- Human
- Clone
- R01-8B1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 22 kDa; Observed MW: 22 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- O-6-Methylguanine-DNA Methyltransferase; Methylated-DNA--Protein-Cysteine Methyltransferase; -O-Methylguanine-DNA Methyltransferase; O-6-Methylguanine-DNA-Alkyltransferase; EC 2.1.1.63; O6-Methylguanine-DNA Methyltransferase; Methylguanine-DNA Methyltransferase
- Gene ID
- 4255
- UniProt ID
- P16455
- Sequence Similarities
- Belongs to the MGMT family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_002403.2
- Function
- Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Recommended Products
Secondary Antibody
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2127z | Mouse Anti-MGMT Recombinant Antibody (clone 35A8) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MRO-2032-CN | Rabbit Anti-MGMT Polyclonal Antibody (MRO-2032-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS13-YC728 | Rabbit Anti-MGMT Recombinant Antibody (VS13-YC728) | WB, IHC-P | Rabbit IgG |
| VS7-0425-WR381 | Mouse Anti-MGMT Recombinant Antibody (clone 1A11F8) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR382 | Mouse Anti-MGMT Recombinant Antibody (clone 1A11A8) | WB, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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