Recombinant Rabbit Anti-MIF Capture Antibody (clone R02-1N7)
CAT#: VS3-WK1750
This antibody is a rabbit recombinant antibody against human MIF. It was developed by immunization of rabbit with recombinant human MIF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R02-1N7 and R07-1X2. The clone R02-1N7 can be used as a capture antibody in ELISA analysis to identify human MIF.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
Normal Tissue
Figure 4 Prostate
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
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Specifications
- Immunogen
- Recombinant human MIF protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MIF
- Species Reactivity
- Human
- Clone
- R02-1N7
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Macrophage Migration Inhibitory Factor; Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor); Glycosylation-Inhibiting Factor; Phenylpyruvate Tautomerase; L-Dopachrome Tautomerase; L-Dopachrome Isomerase
- Gene ID
- 4282
- UniProt ID
- P14174
- Long Name
- Macrophage Migration Inhibitory Factor
- Sequence Similarities
- Belongs to the MIF family.
- Cellular Localization
- Cytoplasm, Secreted
- Protein Refseq
- NP_002406.1
- Function
- Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1484z | Mouse Anti-MIF Recombinant Antibody (clone 19G7) | WB, ELISA, IHC | Mouse IgG1, κ |
| MOB-1923MZ | Recombinant Mouse Anti-Human MIF Antibody (clone 2A10-4D3) | IHC-P, WB | Mouse antibody |
| HPAB-1327LY | Human Anti-MIF Recombinant Antibody (HPAB-1327LY) | Inhib, ELISA | Human IgG |
| VS3-WK1846 | Recombinant Rabbit Anti-MIF Capture Antibody (clone R43-5M-6) | ELISA | Rabbit IgG |
| VS3-WK1847 | Recombinant Rabbit Anti-MIF Detection Antibody (clone R85-5I-4) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H41 | Anti-Human MIF Recombinant Antibody (TAB-H41) | FuncS, IF, Neut, ELISA, FC, IP, IHC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0806CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0806CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0807CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0807CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0808CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0808CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0809CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0809CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0810CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0810CL-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1741CQ | Recombinant Mouse Anti-MIF Antibody (NEUT-1741CQ) | ELISA(Cap), WB, Neut | IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2234 | Hi-Affi™ Recombinant Rabbit Anti-MIF Monoclonal Antibody (DS2234AB) | WB, ICC, IF, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0104-YC-F(E) | Human Anti-MIF Recombinant Antibody; Fab Fragment (HPAB-0104-YC-F(E)) | ELISA, Activ | Human Fab |
| HPAB-1311-FY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1311-FY-S(P)) | Inhib | Human scFv |
| HPAB-1312-FY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1312-FY-S(P)) | Inhib | Human scFv |
| HPAB-1313-FY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1313-FY-S(P)) | Inhib | Human scFv |
| HPAB-1314-FY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1314-FY-S(P)) | Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1328LY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1328LY-S(P)) | Inhib, ELISA | Human scfv |
| HPAB-1329LY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1329LY-S(P)) | Inhib, ELISA | Human scfv |
| HPAB-1330LY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1330LY-S(P)) | Inhib, ELISA | Human scfv |
| HPAB-1331LY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1331LY-S(P)) | Inhib, ELISA | Human scfv |
| HPAB-1332LY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1332LY-S(P)) | Inhib, ELISA | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H41 | Afuco™ Anti-MIF ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H41) | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC173 | AbPlus™ Anti-MIF Magnetic Beads (VS-0724-YC173) | IP, Protein Purification | |
| VS-0724-YC174 | AbPlus™ Anti-Mif Magnetic Beads (VS-0724-YC174) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY149 | Human Anti-MIF scFv-Fc Chimera (VS-0425-FY149) | FC, ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4394 | Anti-MIF Immunohistochemistry Kit | IHC | |
| VS-0525-XY4395 | Anti-Mouse MIF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC222 | Recombinant Anti-MIF Eliminating Antibody, pH-Sensitive (VS-0625-YC222) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
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