Recombinant Rabbit Anti-MIF Capture Antibody (clone R43-5M-6) (CAT#: VS3-WK1846)
This antibody is a rabbit recombinant antibody against mouse MIF. It was developed by immunization of rabbit with recombinant mouse MIF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R43-5M-6 and R85-5I-4. The clone R43-5M-6 can be used as a capture antibody in ELISA analysis to identify mouse MIF.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
Figure 2 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
Figure 4 Prostate
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HEK93)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
Specifications
- Immunogen
- Recombinant mouse MIF protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse MIF
- Species Reactivity
- Mouse
- Clone
- R43-5M-6
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- GIF; DER6; Glif
- Gene ID
- 17319
- UniProt ID
- P34884
- Long Name
- Macrophage migration inhibitory factor (glycosylation-inhibiting factor)
- Sequence Similarities
- Belongs to the MIF family.
- Cellular Localization
- Cytoplasm, Secreted
- Protein Refseq
- NP_034928.1
- Function
- Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (By similarity).
The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (By similarity).
Counteracts the anti-inflammatory activity of glucocorticoids (By similarity).
Recommended Products
Detection antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Human Antibody Products
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ScFv Antibody Products
Fab Antibody Products
ADCC Enhanced Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H41 | Anti-Human MIF Recombinant Antibody (TAB-H41) | FuncS, IF, Neut, ELISA, FC, IP, IHC | IgG1 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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