Recombinant Rabbit Anti-MIF Detection Antibody (clone R85-5I-4) (CAT#: VS3-WK1847)
This antibody is a rabbit recombinant antibody against mouse MIF. It was developed by immunization of rabbit with recombinant mouse MIF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R43-5M-6 and R85-5I-4 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R85-5I-4 can be used in ELISA analysis to identify mouse MIF.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
Figure 2 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
Figure 4 Prostate
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HEK93)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
Specifications
- Immunogen
- Recombinant mouse MIF protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse MIF
- Species Reactivity
- Mouse
- Clone
- R85-5I-4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- GIF; DER6; Glif
- Gene ID
- 17319
- UniProt ID
- P34884
- Long Name
- Macrophage migration inhibitory factor (glycosylation-inhibiting factor)
- Sequence Similarities
- Belongs to the MIF family.
- Cellular Localization
- Cytoplasm, Secreted
- Protein Refseq
- NP_034928.1
- Function
- Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (By similarity).
The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (By similarity).
Counteracts the anti-inflammatory activity of glucocorticoids (By similarity).
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1484z | Mouse Anti-MIF Recombinant Antibody (clone 19G7) | WB, ELISA, IHC | Mouse IgG1, κ |
| HPAB-1327LY | Human Anti-MIF Recombinant Antibody (HPAB-1327LY) | Inhib, ELISA | Human IgG |
| HPAB-1328LY | Human Anti-MIF Recombinant Antibody (HPAB-1328LY) | Inhib, ELISA | Human IgG |
| ZG-0468U | Rabbit Anti-MIF Recombinant Antibody (clone 7A9) | ELISA, WB | Rabbit IgG |
| VS3-FY928 | Recombinant Rabbit Anti-MIF Antibody (clone R05-8H5) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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