Recombinant Mouse Anti-MTDH Antibody (clone 1F3A3) (CAT#: VS3-FY969)
This product is a recombinant mouse antibody that recognizes MTDH. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry. The clone 1F3A3 is specific for MTDH.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/1894_H7_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/1894_H7_3_selected.jpg
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_4_2_selected.jpg
(Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_4_2_selected.jpg
Figure 4 IHC staining of human cerebral corte
(Immunohistochemical staining of human cerebral cortex shows strong positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/ihc_selected.jpg
Figure 5 IHC staining of human smooth muscle
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68205/ihc_selected.jpg
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68205/ihc_selected.jpg
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_3.jpg
Figure 7 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_5.jpg
Figure 9 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_6_6.jpg
Figure 10 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_8_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_8_8.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147649-MTDH
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147649-MTDH
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Clone
- 1F3A3
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry.
Target
- Alternative Names
- Metadherin; Lysine-Rich CEACAM1 Co-Isolated Protein; Astrocyte Elevated Gene-1 Protein; Metastasis Adhesion Protein; Astrocyte Elevated Gene 1; 3D3/LYRIC; AEG-1
- Gene ID
- 92140
- UniProt ID
- Q86UE4
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-678-F(E) | Recombinant Anti-human MTDH Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-678-F(E) | Recombinant Human Anti-human MTDH Antibody Fab Fragment | WB, IF, FuncS | Fab |
| HPAB-0497-CN-F(E) | Mouse Anti-MTDH Recombinant Antibody (clone 1E3); Fab Fragment | WB, ELISA, IHC | Mouse Fab |
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