Recombinant Mouse Anti-Mycoplasma p37 protein Antibody (CA27) (CAT#: HPAB-0208-YJ)
This product is a recombinant mouse antibody that recognizes Mycoplasma p37 protein and blood cancer cells infected with mycoplasma.
Specific Inquiry
FC
Figure 1 CA27 recognizes an approximately 40 kDa surface protein on A549 and HepG2 cells.
Flow cytometric analysis of H358, A549, HepG2, and PBMCs with CA27.
Choi, H. S., Lee, H. M., Kim, W. T., Kim, M. K., Chang, H. J., Lee, H. R., ... & Ryu, C. J. (2014). Detection of mycoplasma infection in circulating tumor cells in patients with hepatocellular carcinoma. Biochemical and biophysical research communications, 446(2), 620-625.
WB
Figure 2 CA27 recognizes an approximately 40 kDa surface protein on A549 and HepG2 cells.
Western blot analysis of CA27 antigen in H358, A549 and HepG2 cells.
Choi, H. S., Lee, H. M., Kim, W. T., Kim, M. K., Chang, H. J., Lee, H. R., ... & Ryu, C. J. (2014). Detection of mycoplasma infection in circulating tumor cells in patients with hepatocellular carcinoma. Biochemical and biophysical research communications, 446(2), 620-625.
ICC
Figure 3 Confocal microscopic analysis of CTCs detected in a patient with HCC.
CA27-postive CTCs were enriched using CA27-conjugated magnetic beads and stained with CA27 (red arrowheads) and anti-CD45 antibodies (green arrowheads). Residual magnetic beads are also shown and nonspecifically stained with DAPI (black arrowheads). Nuclei were stained with DAPI. Two different images (A, B) from patient #06 are shown. Bright field images (BF) are also shown. The scale bar is 7.5 lm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Choi, H. S., Lee, H. M., Kim, W. T., Kim, M. K., Chang, H. J., Lee, H. R., ... & Ryu, C. J. (2014). Detection of mycoplasma infection in circulating tumor cells in patients with hepatocellular carcinoma. Biochemical and biophysical research communications, 446(2), 620-625.
FC
Figure 4 Flow Cytometry.
Mycoplasmas (1x105 CFU/ml) were incubated with PBS (positive control), boiled CA27 (negative control, 3 μg/ml), or active CA27 (1–3 μg/ml), added to Huh7 cells, and then cultured for 2 days. Mycoplasma infection of Huh7 cells was then measured by flow cytometric analysis with CA27.
Kim, M. K., Kim, W. T., Lee, H. M., Choi, H. S., Jo, Y. R., Lee, Y., ... & Jang, Y. J. (2016). Mapping of a Mycoplasma-Neutralizing Epitope on the Mycoplasmal p37 Protein. PloS one, 11(12), e0169091.
Neut
Figure 5 CA27 neutralizes mycoplasma infection.
Graphic presentation of relative CA27 binding to mycoplasma-infected Huh7 cells. MFI (mean fluorescence intensity) values of CA27 binding were measured and presented as percentages.
Kim, M. K., Kim, W. T., Lee, H. M., Choi, H. S., Jo, Y. R., Lee, Y., ... & Jang, Y. J. (2016). Mapping of a Mycoplasma-Neutralizing Epitope on the Mycoplasmal p37 Protein. PloS one, 11(12), e0169091.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG3, κ
- Specificity
- Mycoplasma
- Species Reactivity
- Mycoplasma
- Clone
- CA27
- Applications
- FC, ICC, WB, ELISA, IP
- Related Disease
- Infection; Blood cancer
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry, Immunocytochemistry, Western Blot, ELISA, Immunoprecipitation. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Mycoplasma p37 protein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0208-YJ-S(P) | Recombinant Mouse Anti-Mycoplasma p37 protein Antibody scFv Fragment (CA27) | ELISA, FC, WB, IP, ICC | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0208-YJ-F(E) | Recombinant Mouse Anti-Mycoplasma p37 protein Antibody Fab Fragment (CA27) | ELISA, FC, WB, IP, ICC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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