Recombinant Mouse Anti-Mycoplasma p37 protein Antibody (CA27) (CAT#: HPAB-0208-YJ)
This product is a recombinant mouse antibody that recognizes Mycoplasma p37 protein and blood cancer cells infected with mycoplasma.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
FC
Figure 4 Flow Cytometry.
Mycoplasmas (1x105 CFU/ml) were incubated with PBS (positive control), boiled CA27 (negative control, 3 μg/ml), or active CA27 (1–3 μg/ml), added to Huh7 cells, and then cultured for 2 days. Mycoplasma infection of Huh7 cells was then measured by flow cytometric analysis with CA27.
Kim, M. K., Kim, W. T., Lee, H. M., Choi, H. S., Jo, Y. R., Lee, Y., ... & Jang, Y. J. (2016). Mapping of a Mycoplasma-Neutralizing Epitope on the Mycoplasmal p37 Protein. PloS one, 11(12), e0169091.
Neut
Figure 5 CA27 neutralizes mycoplasma infection.
Graphic presentation of relative CA27 binding to mycoplasma-infected Huh7 cells. MFI (mean fluorescence intensity) values of CA27 binding were measured and presented as percentages.
Kim, M. K., Kim, W. T., Lee, H. M., Choi, H. S., Jo, Y. R., Lee, Y., ... & Jang, Y. J. (2016). Mapping of a Mycoplasma-Neutralizing Epitope on the Mycoplasmal p37 Protein. PloS one, 11(12), e0169091.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG3, κ
- Specificity
- Mycoplasma
- Species Reactivity
- Mycoplasma
- Clone
- CA27
- Applications
- FC, ICC, WB, ELISA, IP
- Related Disease
- Infection; Blood cancer
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry, Immunocytochemistry, Western Blot, ELISA, Immunoprecipitation. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Mycoplasma p37 protein
Related Resources
Zhang, Yibin, et al. "Nucleic acid aptamer controls mycoplasma infection for inhibiting the malignancy of esophageal squamous cell carcinoma." Molecular Therapy 30.6 (2022): 2224-2241. https://doi.org/10.1016/j.ymthe.2022.02.018
This research investigates how Mycoplasma hyorhinis infection influences esophageal squamous cell carcinoma (ESCC) progression and develops a novel therapeutic approach using DNA aptamers. The study demonstrates that M. hyorhinis infection promotes the malignancy of ESCC cells by enhancing migration, invasion, and metastasis capabilities. Using cell-SELEX technology, researchers generated a single-stranded DNA aptamer called ZY3A that specifically targets the M. hyorhinis-encoded p37 protein. The aptamer works by blocking the interaction between p37 protein and its receptor TLR4 on ESCC cell membranes, effectively neutralizing M. hyorhinis infection and inhibiting cancer cell migration, invasion, and metastasis both in vitro and in vivo.
Creative Biolabs provided a monoclonal anti-mycoplasma p37 protein antibody (HPAB-0208-YJ) that was crucial for identifying and confirming the binding target of the aptamer ZY3A. This antibody enabled researchers to validate that p37 was the specific target protein and helped elucidate the mechanism through which the aptamer neutralized M. hyorhinis infection. The antibody's specificity was essential for the pulldown assays, western blot analysis, and immunofluorescence studies that demonstrated how ZY3A blocks the interaction between p37 and TLR4, providing key evidence for developing ZY3A as a potential therapeutic tool against M. hyorhinis-associated ESCC.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0208-YJ-S(P) | Recombinant Mouse Anti-Mycoplasma p37 protein Antibody scFv Fragment (CA27) | ELISA, FC, WB, IP, ICC | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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