Recombinant Mouse Anti-MYOD1 Antibody (clone 3A10-1C10-10D5) (CAT#: VS3-FY997)
This product is a recombinant mouse antibody that recognizes MYOD1. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 3A10-1C10-10D5 is specific for human MYOD1. The antigen is synthetic peptide derived from human myod1.
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IHC-P
Figure 1. Recombinant Mouse Anti-MYOD1 Antibody (clone 3A10-1C10-10D5) in IHC-P.
Immunohistochemical analysis of paraffin-embedded rhabdomyosarcoma using myoblast assay protein 1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
IHC-P
Figure 2. Recombinant Mouse Anti-MYOD1 Antibody (clone 3A10-1C10-10D5) in IHC-P.
Immunohistochemical analysis of paraffin-embedded rhabdomyosarcoma using myoblast assay protein 1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide derived from human MyoD1.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human MYOD1
- Species Reactivity
- Human
- Clone
- 3A10-1C10-10D5
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Myogenic Differentiation 1; Myogenic Factor 3; Class C Basic Helix-Loop-Helix Protein 1; Myoblast Determination Protein 1; BHLHc1; Myf-3; MYF3; MYOD; PUM
- Gene ID
- 4654
- UniProt ID
- P15172
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
- Protein Refseq
- NP_002469.2
- Function
- Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
IgM Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2296z | Mouse Anti-MYOD1 Recombinant Antibody (clone 24G3) | FC, IF, IHC | Mouse IgG1, κ |
| MOB-0329MZ | Recombinant Mouse Anti-Human Myogenic Differentiation 1 Antibody (clone 6.9B) | IHC | Mouse antibody |
| MOB-0131F | Mouse Anti-MyoD1 Recombinant Antibody (MOB-0131F) | IHC-P, IF | Mouse IgG |
| MOB-0132F | Mouse Anti-MyoD1 Recombinant Antibody (MOB-0132F) | IHC-P, IF | Mouse IgG |
| VS3-WK545 | Rabbit Anti-MYOD1 Recombinant Antibody (clone JM10-72) | WB, IP, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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