Recombinant Mouse Anti-NBR1 Antibody (VS4-CJ49)
CAT#: VS4-CJ49
This mouse anti-NBR1 antibody is a product that recognizes human, mouse, rat NBR1. The immunogen of this antibody is a recombinant protein corresponding to NBR1. The mAb has been tested for use in ELISA, Immunohistochemistry (1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22944/1584_E8_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22999/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23999/52902_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23999/52902_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23999/52902_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188554-NBR1
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Specifications
- Immunogen
- Recombinant Protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat NBR1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- IAI3B; M17S2; MIG19; 1A1-3B
- Gene ID
- 4077
- UniProt ID
- Q14596
- Long Name
- NBR1 autophagy cargo receptor
- Cellular Localization
- Cytoplasm, Cytoplasmic vesicle, Lysosome
- Post Translation Modifications
- (Microbial infection) Cleaved by S.pyogenes SpeB protease; leading to its degradation (PubMed:24331465).
Degradation by SpeB prevents autophagy, promoting to S.pyogenes intracellular replication (PubMed:24331465).
Phosphorylated by GSK3A; this phosphorylation inhibits NBR1 involvement in the formation of ubiquitinated protein aggregates.
- Protein Refseq
- NP_001278500.1; NP_001278501.1; NP_005890.2
- Function
- Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed:33577621, PubMed:24692539).
Possesses a double function during the selective autophagy by acting as a shuttle bringing ubiquitinated proteins to autophagosomes and also by participating in the formation of protein aggregates (PubMed:24879152, PubMed:34471133).
Plays a role in the regulation of the innate immune response by modulating type I interferon production and targeting ubiquitinated IRF3 for autophagic degradation (PubMed:35914352).
In response to oxidative stress, promotes an increase in SQSTM1 levels, phosphorylation, and body formation by preventing its autophagic degradation (By similarity).
In turn, activates the KEAP1-NRF2/NFE2L2 antioxidant pathway (By similarity).
Plays also non-autophagy role by mediating the shuttle of IL-12 to late endosome for subsequent secretion (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "NBR1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0023CT | Recombinant Mouse anti-Human NBR1 Monoclonal antibody (EML534) | IHC-P, WB | |
| MOB-0139F | Mouse Anti-NBR1 Recombinant Antibody (clone 7C3) | IF, IHC-P | Mouse IgG |
| VS3-QX803 | Mouse Anti-NBR1 Recombinant Antibody (clone 7H2-4B2-6D6) | IHC | Mouse IgG1 |
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