Recombinant Rabbit Anti-NDUFB4 Antibody (clone R05-5C8)
CAT#: VS3-FY1016
This product is a recombinant rabbit antibody that recognizes NDUFB4. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-5C8 is specific for human, mouse, rat NDUFB4. The antigen is human ndufb4 recombinant protein.
Tested Data
ICC
Figure 1. Recombinant Rabbit Anti-NDUFB4 Antibody (clone R05-5C8) in ICC.
Immunocytochemical analysis of NDUFB4 (green) in HeLa using NDUFB4 antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-NDUFB4 Antibody (clone R05-5C8) in WB.
Western Blot analysis of NDUFB4 in K562, rat brain lysates using NDUFB4 antibody.
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Specifications
- Immunogen
- Human NDUFB4 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat NDUFB4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-5C8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 15 kDa; Observed MW: 15 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Subunit B4; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 4, 15kDa; NADH-Ubiquinone Oxidoreductase B15 Subunit; Complex I B15 Subunit; Complex I-B15; CI-B15; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 4 (15kD, B15); NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 4; B15
- Gene ID
- 4710
- UniProt ID
- O95168
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NDUFB4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4742 | Anti-NDUFB4 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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