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NDUFS1
Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2)

Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2) (CAT#: VS3-FY1017)

Name: Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2)
SKU: VS3-FY1017
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes NDUFS1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-3I2 is specific for human, mouse, rat NDUFS1. The antigen is human ndufs1 synthetic peptide.

Tested Data
Gene Expression
Datasheet
MSDS
COA

ICC

Figure 1. Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2) in ICC.

Figure 1. Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2) in ICC.

Immunocytochemical analysis of NDUFS1 (green) in HeLa using NDUFS1 antibody and DAPI (blue).

ICC

Figure 2. Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2) in ICC.

Figure 2. Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2) in ICC.

Immunocytochemical analysis of NDUFS1 (green) in HeLa using NDUFS1 antibody and DAPI (blue).

Figure 3. Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2) in WB.

Figure 3. Recombinant Rabbit Anti-NDUFS1 Antibody (clone R02-3I2) in WB.

Western Blot analysis of Ndufs1 in mouse heart, mouse kidney, rat skeletal muscle lysates using Ndufs1 Antibody.

Subcellular Location

Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64605/if_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70844/153348_B_8_5.jpg

Figure 3 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70844/153348_A_8_5.jpg

Figure 4 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70844/153348_A_5_6.jpg

Figure 5 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70844/153348_B_6_6.jpg

RNA Expression

Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000023228-NDUFS1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human Ndufs1 synthetic peptide.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat NDUFS1

Species Reactivity
Human, Mouse, Rat

Clone
R02-3I2

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 79 kDa; Observed MW: 79 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
NADH:Ubiquinone Oxidoreductase Core Subunit S1; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase); NADH-Ubiquinone Oxidoreductase 75 KDa Subunit, Mitochondrial; Complex I 75kDa Subunit; CI-75Kd; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1 (75kD) (NADH-Coenzyme Q Reductase); Mitochondrial NADH-Ubiquinone Oxidoreductase 75 KDa Subunit

Gene ID
4719

UniProt ID
P28331

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody Products Antibody Magnetic Beads

CAT	Product Name	Application	Type
MOR-2401	Hi-Affi™ Recombinant Rabbit Anti-NDUFS1 Monoclonal Antibody (DS2401AB)	WB, FC, ICC/IF, IHC-P	IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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