Recombinant Rabbit Anti-NDUFS2 Antibody (clone R08-4D7)

Name: Recombinant Rabbit Anti-NDUFS2 Antibody (clone R08-4D7)
SKU: VS3-FY1018
Rating: 5 (1 reviews)

CAT#: VS3-FY1018

This product is a recombinant rabbit antibody that recognizes NDUFS2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R08-4D7 is specific for human NDUFS2. The antigen is human ndufs2 recombinant protein.

Gene Expression Tested Data

Subcellular Location Figure 1 IF staining of human cell line HeLa

Immunofluorescent staining of human cell line HeLa shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55140/881_A3_1_selected.jpg

Normal Tissue Figure 2 Heart muscle

High expression in heart muscle

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55140/128534_B_5_6_val_selected.jpg

Normal Tissue Figure 3 IHC staining of human heart muscle

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61953/146882_B_6_6_selected.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61953/146882_A_8_8.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61953/146882_B_7_5.jpg

Normal Tissue Figure 6 Colon

Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61953/146882_A_8_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61953/146882_A_9_4.jpg

Normal Tissue Figure 8 Kidney

Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61953/146882_A_7_5.jpg

Normal Tissue Figure 9 Testis

Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61953/146882_A_6_6.jpg

RNA Expression Figure 10 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000158864-NDUFS2

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Figure 1. Recombinant Rabbit Anti-NDUFS2 Antibody (clone R08-4D7) in WB.

Western Blot analysis of NDUFS2 in Hela, HepG2, U2OS, U251, HL-60 lysates using NDUFS2 Antibody.

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Figure 2. Recombinant Rabbit Anti-NDUFS2 Antibody (clone R08-4D7) in IHC-P.

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Specifications

Immunogen
Human NDUFS2 recombinant protein.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human NDUFS2

Species Reactivity
Human

Clone
R08-4D7

Applications
Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 53 kDa; Observed MW: 49 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20

Target

Alternative Names
NADH:Ubiquinone Oxidoreductase Core Subunit S2; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase); NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 2, Mitochondrial; NADH-Ubiquinone Oxidoreductase 49 KDa Subunit; Complex I 49kDa Subunit; Complex I-49kD; CI-49kD; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2 (49kD) (NADH-Coenzyme Q Reductase)

Gene ID
4720

UniProt ID
O75306

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-FY1018.pdf

MSDS

MSDS - VS3-FY1018.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Secondary Antibody

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See other products for "NDUFS2"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-3192z	Mouse Anti-NDUFS2 Recombinant Antibody (clone 18B3)	WB, IP, IF, IHC, ELISA	Mouse IgG1, κ
MOB-1324MZ	Recombinant Mouse Anti-Human NDUFS2 Antibody (clone 8B23CF6BE6)	ICC, IF, WB	Mouse antibody

CAT	Product Name	Application
VS-0525-XY4749	Anti-NDUFS2 Immunohistochemistry Kit	IHC
VS-0525-XY4750	Anti-Mouse NDUFS2 Immunohistochemistry Kit	IHC
VS-0525-XY4751	Anti-Rat NDUFS2 Immunohistochemistry Kit	IHC

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