Recombinant Rabbit Anti-NFE2L2 (phospho Ser40) Antibody (clone R03-7C4)
CAT#: VS3-FY1033
This product is a recombinant rabbit antibody that recognizes NFE2L2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R03-7C4 is specific for human NFE2L2. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser40 of human nrf2.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2990/1586_E11_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43438/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2990/8743_A_4_4_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_9_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_4_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000116044-NFE2L2
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WB
Figure 1. Recombinant Rabbit Anti-NFE2L2 (phospho Ser40) Antibody (clone R03-7C4) in WB.
Western Blot analysis of Phospho-Nrf2 (Ser40) in HeLa lysate K562 using Phospho-Nrf2 (Ser40) Antibody.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser40 of human Nrf2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NFE2L2
- Species Reactivity
- Human
- Clone
- R03-7C4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 68 kDa; Observed MW: 100 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NFE2L2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2432 | Hi-Affi™ Recombinant Rabbit Anti-NFE2L2 Monoclonal Antibody (DS2432AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2085-CN | Rabbit Anti-NFE2L2 Polyclonal Antibody (MRO-2085-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2086-CN | Rabbit Anti-NFE2L2 Polyclonal Antibody (MRO-2086-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0014U | Rabbit Anti-NFE2L2 Recombinant Antibody (clone 2D12) | ELISA, IF | Rabbit IgG |
| VS3-QX807 | Mouse Anti-NFE2L2 Recombinant Antibody (clone 5F10-G4-B3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS4-CJ53 | Recombinant Mouse Anti-NFE2L2 Antibody (clone 2F6C6) | ELISA, WB, IHC, IF, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4809 | Anti-NFE2L2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4810 | Anti-Mouse NFE2L2 Immunohistochemistry Kit | IHC |
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