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NFE2L2
Recombinant Rabbit Anti-NFE2L2 (phospho Ser40) Antibody (clone R03-7C4)

Recombinant Rabbit Anti-NFE2L2 (phospho Ser40) Antibody (clone R03-7C4) (CAT#: VS3-FY1033)

Name: Recombinant Rabbit Anti-NFE2L2 (phospho Ser40) Antibody (clone R03-7C4)
SKU: VS3-FY1033
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes NFE2L2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R03-7C4 is specific for human NFE2L2. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser40 of human nrf2.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-NFE2L2 (phospho Ser40) Antibody (clone R03-7C4) in WB.

Figure 1. Recombinant Rabbit Anti-NFE2L2 (phospho Ser40) Antibody (clone R03-7C4) in WB.

Western Blot analysis of Phospho-Nrf2 (Ser40) in HeLa lysate K562 using Phospho-Nrf2 (Ser40) Antibody.

Subcellular Location

Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2990/1586_E11_3_selected.jpg

Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43438/if_selected.jpg

Normal Tissue

Figure 3 IHC staining of human gallbladder
(Immunohistochemical staining of human gallbladder shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2990/8743_A_4_4_selected.jpg

Figure 4 Lymph node
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_9_8.jpg

Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_B_8_5.jpg

Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_8_3.jpg

Figure 7 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_7_4.jpg

Figure 8 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_7_5.jpg

Figure 9 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_4_6.jpg

RNA Expression

Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000116044-NFE2L2

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser40 of human Nrf2.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human NFE2L2

Species Reactivity
Human

Clone
R03-7C4

Applications
Western Blot, Immunohistochemistry-Paraffin

Conjugate
Unconjugated

MW
Calculated MW: 68 kDa; Observed MW: 100 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100

Target

Alternative Names
Nuclear Factor, Erythroid 2 Like 2; NF-E2-Related Factor 2; HEBP1; NRF2; Nuclear Factor (Erythroid-Derived 2)-Like 2; Nuclear Factor Erythroid 2-Related Factor 2

Gene ID
4780

UniProt ID
Q16236

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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MOB-3994z	Mouse Anti-NFE2L2 Recombinant Antibody (clone 14H9)	IHC, WB, ELISA	Mouse IgG1
MOB-1458CT	Recombinant Mouse anti-Human NFE2L2 Monoclonal antibody (2G7)	ELISA, WB
ZG-0014U	Rabbit Anti-NFE2L2 Recombinant Antibody (clone 2D12)	ELISA, IF	Rabbit IgG
VS4-CJ53	Recombinant Mouse Anti-NFE2L2 Antibody (clone 2F6C6)	ELISA, WB, IHC, IF, FC	Mouse IgG2b
VS3-XY3445	Rabbit Anti-NFE2L2 Recombinant Antibody (clone R04-6H3)	WB, IHC-P, ICC, IF, IP, ChIP	Rabbit IgG

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