Recombinant Mouse Anti-NFKBIB Antibody (VS4-CJ55)
CAT#: VS4-CJ55
This mouse anti-NFKBIB antibody is a product that recognizes human, mouse, rat NFKBIB. The immunogen of this antibody is a recombinant protein corresponding to NFKBIB. The mAb has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63734/1139_C10_2_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000104825-NFKBIB
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Specifications
- Immunogen
- Recombinant Protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat NFKBIB
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).
Target
- Alternative Names
- IKBB; TRIP9
- Gene ID
- 4793
- UniProt ID
- Q15653
- Long Name
- NFKB inhibitor beta
- Sequence Similarities
- Belongs to the NF-kappa-B inhibitor family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
- Protein Refseq
- NP_001230045.1; NP_002494.2
- Function
- Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "NFKBIB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-485MZ | Human Anti-NFKBIB Recombinant Antibody (TAB-485MZ) | Cyt, FC, WB | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-485MZ-S(P) | Human Anti-NFKBIB Recombinant Antibody; scFv Fragment (TAB-485MZ-S(P)) | FC, Confocal microscopy, WB, IP | Human scFv |
| TAB-485MZ-F(E) | Human Anti-NFKBIB Recombinant Antibody; Fab Fragment (TAB-485MZ-F(E)) | FC, Confocal microscopy, WB, IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0882CT | Recombinant Mouse anti-Human NFKBIB Monoclonal antibody (EML735) | WB | |
| VS3-QX814 | Mouse Anti-NFKBIB Recombinant Antibody (clone 3H3-6E9-9H9) | IHC | Mouse IgG1 |
| VS3-QX815 | Mouse Anti-NFKBIB Recombinant Antibody (clone 5H3-4C7-1H6) | IHC | Mouse IgG1 |
| VS3-WK988 | Mouse Anti-NFKBIB Recombinant Antibody (clone 1F3) | IHC-P, IF | Mouse IgG |
| VS3-WK990 | Rabbit Anti-NFKBIB Recombinant Antibody (VS3-WK990) | WB, ICC, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2436 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIB Monoclonal Antibody (DS2436AB) | ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1227 | AbPlus™ Anti-NFKBIB Magnetic Beads (VS-0724-YC1227) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4823 | Anti-NFKBIB Immunohistochemistry Kit | IHC |
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