Recombinant Rabbit Anti-NODAL Antibody (clone R06-5A3)
CAT#: VS3-FY1047
This product is a recombinant rabbit antibody that recognizes NODAL. This antibody has been reported for use in Western Blot. The clone R06-5A3 is specific for human, mouse, rat NODAL. The antigen is human nodal synthetic peptide.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45201/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45201/100225_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45201/100225_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45201/100225_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45201/100225_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (CACO-2, SH-SY5Y, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156574-NODAL
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WB
Figure 1. Recombinant Rabbit Anti-NODAL Antibody (clone R06-5A3) in WB.
Western Blot analysis of Nodal in C6, 3T3, Hela lysates using Nodal Antibody.
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Specifications
- Immunogen
- Human Nodal Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat NODAL
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-5A3
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 40 kDa; Observed MW: 40 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NODAL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0792CT | Recombinant Mouse anti-Human NODAL Monoclonal antibody (EML676) | FC, IHC-P, WB | |
| VS13-YC822 | Rabbit Anti-NODAL Recombinant Antibody (VS13-YC822) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2462 | Hi-Affi™ Recombinant Rabbit Anti-NODAL Monoclonal Antibody (DS2462AB) | ICC, IF, IHC-P, WB | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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