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    Recombinant Rabbit Anti-NONO Antibody (clone R05-1F8) (CAT#: VS3-FY1048)

    This product is a recombinant rabbit antibody that recognizes NONO. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-1F8 is specific for human, mouse, rat NONO. The antigen is synthetic peptide of human nmt55/p54nrb.

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    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/877_A7_2_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human skeletal muscle
    Figure 2 IHC staining of human skeletal muscle
    (Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity and additional strong cytoplasmic staining.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/22069/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75% Peripheral nerve/ganglion Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_6_6.jpg
    Figure 8 Ovary
    Figure 8 Ovary
    (Follicle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_5_7.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Low cell line specificity
    Figure 10 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147140-NONO

    Specifications

    • Immunogen
    • Synthetic peptide of human NMT55/p54nrb.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse, Rat NONO
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R05-1F8
    • Applications
    • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
    • Conjugate
    • Unconjugated
    • MW
    • Calculated MW: 54 kDa; Observed MW: 54 kDa

    Product Property

    • Purification
    • Affinity Purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Buffer
    • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
    • Preservative
    • 0.01% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000
      Immunohistochemistry-Paraffin: 1/50-1/100
      Immunofluorescence: 1/50-1/200

    Target

    • Alternative Names
    • Non-POU Domain Containing Octamer Binding; Protein Phosphatase 1, Regulatory Subunit 114; DNA-Binding P52/P100 Complex, 52 KDa Subunit; 54 KDa Nuclear RNA- And DNA-Binding Protein; 55 KDa Nuclear Protein; P54(Nrb); P54NRB; NMT55; NRB54; Non-Pou Domain-Containing Octamer (ATGCAAAT) Binding Protein

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "NONO"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibody Products IgM Antibody Products Human Antibody Products Rabbit Monoclonal Antibody Products ScFv Antibody Products Fab Antibody Products
    CAT Product Name Application Type
    MOB-3007z Mouse Anti-NONO Recombinant Antibody (clone 24H3) WB, IF, IHC Mouse IgG1
    HPAB-0766-CN Human Anti-NONO Recombinant Antibody (HPAB-0766-CN) WB, ELISA, IHC, IP Human IgG
    MRO-1154-CN Recombinant Rabbit Anti-NONO Monoclonal Antibody (JG87-38) WB, IF, IHC Rabbit IgG
    ZG-0061U Rabbit Anti-NONO Recombinant Antibody (clone 8C11) ELISA, WB, IHC, IF Rabbit IgG
    VS13-YC824 Rabbit Anti-NONO Recombinant Antibody (VS13-YC824) WB, IHC-Fr, IHC-P, ICC, IF Rabbit IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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